Effect of inoculation density on maturation of somatic embryos of plantain cv. ‘FHIA-21’ (Musa AAAB)
Journal Title: Biotecnología Vegetal - Year 2014, Vol 14, Issue 2
Abstract
The establishment of conditions of culture is essential to regulate of efficient way the regeneration of plantain plants cv. ‘FHIA-21’ by somatic embryogenesis. It is for that reason that this investigation had like objective to determine the effect of the density of inoculation on maturation of somatic embryos. For the study were added 0.2, 0.4 and 0.6 grams of somatic embryos on 60 ml of culture means maturation semisolid. The evaluations were realized to the 30 days of culture with the analysis of the morphologic characteristics of the embryos, the determination of the fresh mass (g) and presence of embryos with exhibition of apical shoot. Later, the number of germinated embryos was determined and a morphologic characterization of its germination was realized. The results showed the influence of the density of inoculation in the increase of the fresh mass and in the presence of embryos with exhibition of apical shoot. Nevertheless, significant differences with respect to the total of germinated embryos did not appear, the values oscillated between 8.4 and 11.2 by bottle of culture. In spite of it, one better germination was evident when the 0.4 embryos were worked to gMF, during the phase of maturation. From the embryos with complete and partial germination plants were developed to the 25 and 35 days of culture, respectively, during the phase of growth. These plants were characterized to have a height between 3.5 and 4.0cm, more than three open leaves and a system to radical developed. Aspects that favoured a high survival during the phase of acclimatization in green house.
Authors and Affiliations
Harol González Gallardo, Leyanis García-Águila, Rafael G. Kosky, Alexis Rodríguez, Blanca Pérez, Eloísa Rodríguez
Keywords
Related Articles
- EP ID EP683560
- DOI -
- Views 141
- Downloads 0
Extraction of total RNA in plants and filamentous fungi
RNA of high quality is a common element required as starting material in the majority of the techniques used in molecular studies either in plants or in phytopathogenic fungi. In this manuscript we emphasized in the ch...
Effect of the main physicochemical parameters on the somatic embryogenesis process at the bioreactor scale
The bioreactors has been mainly developed for the production of biomass, for that that the glasses for the culture of the different vegetable species with propagation ends, they have had to be adapted in function of the...
Effect of artificial inoculation of Mycosphaerella fijiensis on the induction of defence-related enzymes in two Musa genotypes
Black Sigatoka, caused by Mycosphaerella fijiensis Morelet (anamorph: Pseudocercospora fijiensis (Morelet) Deighton), is regarded as the most economically important foliar disease of bananas and plantains worldwide. Alth...
Epiphytic mycobiota and fungal contaminants from the in vitro establishment of guava (Psidium guajava L)
The studying of contaminats micobiote on guajaba (Psidium guajava L.) could help for creating schedule treatment of donator plants and its explants to eliminate or prevent the fungi contamination during guajaba micropr...
Evaluation of the knowledge of a population about the risk in the case of plantain and banana transformed with genes for resistance to the black sigatoka
An increasing impact of plant biotechnology on society is present in all over the world. Scientific understanding should be developed on people so they can be able to make right personal decisions and participate in pub...