Effect of the main physicochemical parameters on the somatic embryogenesis process at the bioreactor scale
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 4
Abstract
The bioreactors has been mainly developed for the production of biomass, for that that the glasses for the culture of the different vegetable species with propagation ends, they have had to be adapted in function of the specific requirements of each process and cultivation. This way and because a universal team doesnt exist for all the applications, the bioreactors has been object of modifications in their components in dependence of the requirements of each species. He has also been proven that the internal configuration of the culture glass influences in a decisive way on the production and later development of the somatic embryos. Therefore, it is necessary to solve the current technological limitations and to study the effect of the main culture parameters to be able to use this technology type like an alternative for the mass propagation of plants. Different systems have been evaluated of agitation-aeration and designs have been proven that generate drops hydrodynamic forces inside the culture glass, guaranteeing the quality and viability of the culture in suspension, as well as the formation and multiplication of the somatic embryos. They have been studied for several cultures the effects that cause the main physical-chemical parameters in the propagation via somatic embryogenesis to bioreactors scale. They have been defined methodologies and work strategies that combine this culture parameters and they allow to control and to obtain in way stable productions of somatic embryos able to germinate and to transform into plants and these results definitively will allow to take to commercial scale the employment of this technology for the propagation in vitro of many species of economic interest.
Authors and Affiliations
Manuel de Feria
Keywords
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