Effect of Invitro Zinc (II) supplementation on Normal and Cancer Cell lines
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 1
Abstract
Zinc is the most abundant trace element which has role in genetic stability and function, present in the cell nucleus, nucleolus and chromosomes, and stabilizes the structure of DNA, RNA and ribosomes and found in many Zinc binding proteins. The purpose of this study is to access the Zinc cytotoxicity, total cellular Zinc content and total Zinquin Acid (Fluorophore) interaction with cellular Zinc in response to six different Zinc gradient medium using MTT assay, Atomic absorption spectroscopy and Fluorescence spectroscopy respectively. Statistical analysis one way ANOVA and Tukey HSD test for mean comparison was performed. Our findings are, significant differences on normal cell line survival of 108.7% at 10 µM supplemented medium was seen. Cytotoxicity was seen at 80 µM of 89.78% and 86.46% in MDA-MB-231 and U-87-MG Cell lines respectively. Significant increase in cellular influx and accumulation of Zn2+ ions was observed in all cell lines. Fluorescence intensity emission peak response increased with supplementation of gradient medium, which supported the hypothesis of increased cellular Zinc accumulation, λ-max shifting to shorter wavelength i.e. idea supports blue shift emission of Zinquin Acid which interacts not only with free labile Zinc ions but with Zinc bound proteins. Zinc based fluorescence probes help detection of altered expression of Zinc.
Authors and Affiliations
Sandeep Adhikari, Basanta Lamichhane, Pritish Shrestha, Bhupal Govinda Shrestha
Keywords
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