Effect of L- Carnitine and COQ10 Addition to SMARTProMedium on Human SpermConcentration,SpermMorphology and Chromatin StructureduringIn VitroSperm Activation
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 2
Abstract
This study was designed to assess the effect of different concentrations of L-carnitine and Co enzyme Q10 (COQ10) supplied to SMART Pro- medium on human sperm concentration, sperm morphology and chromatin structure during in vitro sperm activation. Eighty seven samples of semen of infertile and fertile men were randomly collected at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/Al- Nahrain University. The mean age of the men was (32.034 ± 0.57) years with mean duration of infertility (4.644 ± 0.25) years. Seminal fluid analyses were done involving macroscopic and microscopic examinations according to WHO criteria. Swim-up technique was dependent for in vitro sperm activation; the washed samples were divided before using centrifugation swim-up technique into 3 groups: control group (G1) without L-carnitine and CoQ10. While, in G2 and G3 two concentrations of L-carnitine (20µg, 40µg) and CoQ10 (20µg, 40µg) were used respectively. Moreover, all groups within post-activation enriched with SMART- Pro media. The sperm concentration, sperm morphology and chromatin structure were evaluated after the addition of L-carnitine and Co Q10. The results showed a Significant (P<0.05) differences in sperm parameters of post-activation when compared to the pre-activation. The results for all semen samples of post activation revealed a significant (P< 0.05) decrease in sperm concentration while, a significant (P<0.05) increase in the percentage of normal sperm morphology as compared to pre- activation. The percentage of normal sperm morphology in 40µg (G3) group showed significant (P<0.05) increase as compared to control group (G1) with no significant (P>0.05) differences with 20µg (G2) group. However, 40µg (G3) group provides improvement in the percentages of normal sperm morphology as compared with 20µg (G2) group with no significant (P>0.05) between G2 and G3. A significant (P<0.05) decrease in fragmented sperm DNA percentage of post activation was seen when compared with the pre-activation with non-significant (P>0.05) differences between G2 and G3. Moreover, DNA fragmentation in the spermatozoa treated by 40µg L-carnitine and CoQ10 was the lowest among post-activation groups. From the results of the study, it can be concluded that the addition of 40 µg of L-carnitine and CoQ10 to washed sperms can improve sperm concentration and normal sperm morphology in vitro.
Authors and Affiliations
Majida H. Mutlag Al-Shammari1, Jabbar H. Yenzeel, Muhammad-Baqir M-R Fakhrildin2
Keywords
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- EP ID EP388817
- DOI 10.9790/3008-1202045155
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