Effect of light and gibberellic acid on in vitro germination of Capsicum annuum L. cv. ‘Papri King’
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 3
Abstract
In the coastal zone of Peru, paprika (Capsicum annuum L.) is a crop of great importance, but it has a high production cost because the seeds are germinated in seedlings and this stage is considered crucial. The objective of the present investigation was to determine the effect of light and gibberellic acid on the in vitro germination of paprika seeds cv. ‘Papri King’. The seeds were disinfected with 70% alcohol for 1 min and then sodium hypochlorite (0.5, 1.0, 1.5 and 2.0%) during 10 minutes. For germination it were placed in MS culture medium. Treatments were formed with the addition of gibberellic acid (AG 3 ) (0.25, 0.5, 1.0 mg l -1 ) and photoperiod of 16 hours light or dark. The percentage of contamination and germination was evaluated in vitro in the first and fourth week and the height (cm) of the seedlings was measured. Using 1.5% and 2% sodium hypochlorite solutions, the seeds were disinfected without microbial contamination. The addition of gibberellic acid and the photoperiod of 16 h light increased the seeds germination. With 1 mg l -1 gibberellic acid and photoperiod of 16 hours light, the highest germination percentage was achieved (97.8%).
Authors and Affiliations
Angel David Hernández Amasifuen, Alexandra Jherina Pineda Lázaro, Hermila Belba Díaz Pillasca
Keywords
Related Articles
- EP ID EP698037
- DOI -
- Views 75
- Downloads 0
Traducción de texto Texto original 109 / 5000 Resultados de traducción Callus formation in Phaseolus vulgaris L cv. Turrialba- 4 with Thidiazuron and 2,4-dichlorophenoxyacetic acid
This work had like objective to obtain callus formation with embryogenic structure in Phaseolus vulgaris L. cv. Turrialba-4 with growth regulators Thidiazuron (TDZ) and 2,4-dichlorophenoxiacetic acid (2,4-D). Mature coty...
Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse
Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on th...
Rooting and acclimatization of outbreaks of Phaseolus vulgaris L. regenerated in vitro
The genus Phaseolus showed to be recalcitrant to in vitro plant generation, which is a prerequisite to start a breeding program. Influence of IBA in the culture medium and the shoot longitude were studied in order to a...
Chemical control of weeds during the acclimatization of in vitro sugarcane plants cv. ‘CP52-43’
Control of weeds in the acclimatization phase of in vitro sugarcane (Saccharum spp.) plants is done manually which raises the cost of production. In order to determine the effectiveness of the chemical control of weeds...
Effect of the intercellular extracts of banana leaves inoculated with Mycosphaerella fijiensis Morelet, on the electronic transport in chloroplasts of the cv Grande naine (AAA)
Some foliar pathogens colonize intercellular spaces of damage tissues during infection process, mediated by toxins production and diffusion to kill adjacent healthy cells. Due to the absence of reliable bioassays, the ph...