Effect of Monoclonal Antibody to Human Zona Pellucida 3 in Luteineizing Hormone Receptor Expression, Estradiol Levels and de Graaf Follicles Quantity in Mice
Journal Title: Turkish Journal of Immunology - Year 2018, Vol 6, Issue 2
Abstract
Objective: This research aimed to evaluate effect of monoclonal antibody to human Zona Pellucida 3 (Mab-hZP3) on luteineizing hormone (LH) receptor expression, estradiol levels and de Graaf follicles quantity of mus musculus mice ovaries. Materials and Methods: True experiment post test only control group design was used as research method. Treatments used were control (adjuvant in Tris HCl), and Mab-hZP3 with dosages of 20 μg, 40 μg and 60 μg which tested on day 10, 15 and 20. Luteineizing hormone (LH) receptor, estradiol level and the number of de Graaf follicles measurement were done by immunohistochemical test, ELISA method, and hematoxilin eosin staining, respectively. Results: The results showed that there was no significant difference found on Mab-hZP3 interaction between 20 μg-60 μg dosages and 10–20 days of observation time towards LH receptor expression, estradiol level and and the number of de Graaf follicles. This was related to specificity of monoclonal antibody used. Conclusion: Mab-hZP3 did not lower LH receptor expression, estradiol level and the number of de Graaf follicles quantity, which means that Mab-hZP3 did not interfere at folliculogenesis and not altering hormones profile. It can be concluded that Mab-hZP3 has the potential to be a safe immnocontraception material. Keywords: de Graff follicles, estradiol, immunocontraception, luteineizing hormone, Mab-hZP3.
Authors and Affiliations
Dintya IVANTARINA, Sanarto SANTOSO, Sutrisno SUTRISNO
Keywords
Related Articles
- EP ID EP451031
- DOI 10.25002/tji.2018.463
- Views 83
- Downloads 0
The Association Between Vitamin D Levels and Infections in Patients with Primary Immunodeficiency
ntroduction: Vitamin D is a hormone responsible in the regulation of immune response. This study was designed to assess the relationship of vitamin D deficiency on the development of infections in patients with primary i...
Expression of Tumor Necrosis Factor-α and Interleukin-6 in Chronic Suppurative Otitis Media
Introduction: Chronic suppurative otitis media (CSOM) with cholesteatoma is decribed by keratin epithelial invasion in the middle ear and osteolysis in both of the ear bones and the temporal bone. Some inflammatory facto...
Effect of Monoclonal Antibody to Human Zone Pellucida 3 on Bone Morphogenetic Protein 15 Expression and Number of Preantral and Antral Follicles in Ovary of Mice
Introduction: Immunocontraception of zone pellucide (ZP3) is known causing disturbance of gap junction which inhibits transport growth factor such as bone morphogenetic protein (BMP-15). Decreased BMP-15 generates distur...
Identification of Hemagglutinin Protein from Streptococcus pneumoniae Pili as a Vaccine Candidate by Proteomic Analysis
Introduction: Streptococcus pneumoniae is a Gram-positive, bacterial human pathogen that causes a large number of respiratory tract infections, such as pneumonia and sinusitis, as well as invasive diseases such as septic...
The Effect of Lactobacillus reuteri on the Percentage of Th17 Cells and Level of IL-17 in Staphylococcus aureus-Induced Puerperal Infection BALB/c Mice
Background: Puerperal infection is an infection of the reproductive tract during labor or puerperal period, which is largely caused by Staphylococcus aureus infection resulting in Toxic Shock Syndrome (TSS). The prevalen...