Effect of nanocarriers with antisense oligonucleotides on histological structure of organs synthesizing prion-protein
Journal Title: Біологія тварин - Year 2016, Vol 18, Issue 3
Abstract
Polymer compounds serves as a perspective carriers for medicinal preparations and engages antisense therapy against diseases with diverse etiology. However, the problem is in toxic effect of polymers on the animal organism. Of particular relevance acquire research on developing effective carriers of nucleotides, which could be used for prevention and treatment for disease of pathological protein synthesis. We have been investigated the transportation and delivery of complexes of oligonucleotides with three newly polymer carriers based on dymetylaminoetylmethakrylate — MP-27, MP-2 and MP-3. The research was conducted by the example of action complexes polymer carriers with antisense oligonucleotides on cellular prion expressions. Nanocarriers influence on the structure of organs of white rats (Rattus norvegicus var. alba, Wistar line) was showed. The complexes with polymers MP-2 and MP-3 caused the minor changes in the microstructure of the brain, small intestine and spleen. In particular, in histological structure of brain, reducing the number of neurons in sight was seen on the 7th day after injection. In the small intestine serous membrane sprawling and Peyer plaques swelling have been detected. Histological preparations spleen revealed an increase in the size of individual follicles. However, the effect of carrier MR-27 does not cause pathological changes in the structure of the small intestine, spleen and brain both separately and in combination with antisense oligonucleotides. So, polymeric carrier MY-27 can be used to transport of medicines.
Authors and Affiliations
N. Yu. Susol, Yu. V. Martyn, N. V. Kuzmina, V. V. Vlizlo
Keywords
Related Articles
- EP ID EP261258
- DOI 10.15407/animbiol18.03.091
- Views 131
- Downloads 0
Phospholipid composition of liver and gills of fish as indicator of surface water rivers of Ternopil region
Growth of human impacts on the aquatic environment exacerbates a problem of survival of organisms under stressful conditions of water environment. Such conditions created by the accumulation of toxic substances. Aquatic...
Analysis of reproduction of sheep of askanian selection
As the objects of research three flocks of Askanian Merino, Askanian Meat-Wool and Askanian Karakul sheep were used. The traditional indicators that characterize the reproductive function in the herd, namely, fertility,...
Nonspecific resistance in piglets' blood with colienterotoxemia and for specific action of gamma globulin
The use specific γ-globulin to increase stress resistance and resistance to colienterotoxemia of piglets at weaning is caused by several factors. According to WHO recommendations, one of the most important areas of appli...
Influence of low doses of taurine on antioxidant defense and lipoprotein composition in rat blood
The aim of our work was to investigate the influence of long-term peroral taurine injection on antioxidant defense and lipoprotein composition in rat blood. Studies were conducted on male 4 months old Wistar rats (n=16)...
The effectiveness of irradiation of bull-sire scrotum with incoherent polarized light
The research was conducted to explore the effect of incoherent polarized light emitted by the lamp “Bioptron Compact III” on the general state of bulls-sires, morphological, biochemical parameters of their blood and qual...