Effect of nanocarriers with antisense oligonucleotides on histological structure of organs synthesizing prion-protein
Journal Title: Біологія тварин - Year 2016, Vol 18, Issue 3
Abstract
Polymer compounds serves as a perspective carriers for medicinal preparations and engages antisense therapy against diseases with diverse etiology. However, the problem is in toxic effect of polymers on the animal organism. Of particular relevance acquire research on developing effective carriers of nucleotides, which could be used for prevention and treatment for disease of pathological protein synthesis. We have been investigated the transportation and delivery of complexes of oligonucleotides with three newly polymer carriers based on dymetylaminoetylmethakrylate — MP-27, MP-2 and MP-3. The research was conducted by the example of action complexes polymer carriers with antisense oligonucleotides on cellular prion expressions. Nanocarriers influence on the structure of organs of white rats (Rattus norvegicus var. alba, Wistar line) was showed. The complexes with polymers MP-2 and MP-3 caused the minor changes in the microstructure of the brain, small intestine and spleen. In particular, in histological structure of brain, reducing the number of neurons in sight was seen on the 7th day after injection. In the small intestine serous membrane sprawling and Peyer plaques swelling have been detected. Histological preparations spleen revealed an increase in the size of individual follicles. However, the effect of carrier MR-27 does not cause pathological changes in the structure of the small intestine, spleen and brain both separately and in combination with antisense oligonucleotides. So, polymeric carrier MY-27 can be used to transport of medicines.
Authors and Affiliations
N. Yu. Susol, Yu. V. Martyn, N. V. Kuzmina, V. V. Vlizlo
Keywords
Related Articles
- EP ID EP261258
- DOI 10.15407/animbiol18.03.091
- Views 127
- Downloads 0
Crossbreeding as element of high-producing dairy cattle
The review article is devoted to questions of the use of crossbreeding in modern dairy cattle. The analysis of process of breed creation in Ukraine during last decades is made. It is shown that during the last 60 years...
The functional condition of the liver in laying hens under hepatosis
The article presents the results of the evaluation of the functional state of a liver in clinical healthy and ill with hepatosis laying hens of the “Loman Brown” breed at age 166, 300 and 530 days. The criterion for eval...
Antiviral activity of novel indole-containing condensed tetracyclic compounds
The article presents the results of determination in vitro of antiviral activity of 50 novel indole-containing condensed tetracyclic compounds relatively Aujeszky’s disease virus and transmissible gastroenteritis in mode...
Dissemination of feline calicivirus desease in megalopolis
The article presents the results of monitoring researches conducted in order to establish the levels of spread of the feline calicivirus disease and the ways of its manifestation in Kyiv during the period of 2012–2016. I...
The content of certain heavy metals in tissues and products of honey-bees under the condition of their feeding with native soy flour adding chloride and aquanano chrome citrate
The influence of feeding with native soy flour, adding chloride and aquanano chrome citrate, on the content of chrome, zink, cuprum, ferrum, cadmium, and plumbum in the tissues of a head, thorax, abdomen and the whole bo...