Effect of Petiveria alliacea Leaves Extract in Decreasing Serum Level of Blood Glucose Level Through Activation of AMPK-α1 in Diabetes Mellitus Rat Models
Journal Title: Indonesian Journal of Clinical Pharmacy - Year 2017, Vol 6, Issue 1
Abstract
Singawalang (Petiveria alliaceae) is a medicinal herb that is used traditionally as folk medicine for various diseases. The plant has been used as an antidiabetic agent in Indonesian society. Until now, the effect and mechanism of action of Petiveria alliaceae as antidiabetic agent is not clear. The aim of the study was to determine the effect of Petiveria alliaceae to blood glucose level and to identify expression of AMPK-α1 in rat liver. The research was experimental study with randomized and was conducted at Laboratory Pharmacology and Therapy, and Laboratory Patology Anatomy, Faculty of Medicine, Universitas Airlangga, on April until August 2015. Twenty five male rats were injected by Streptozotocin to create diabetic rat models and randomly divided into 5 groups. The group 1, 2 and 3 are treatment groups that were given the ethanol extract of Petiveria alliaceae at dose 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw, orally, once daily for fourteen days. The fourth group is a negative control who were given distilled water and the fifth group is a positive control who were given metformin at dose 150 mg/ kgbw. On the 15th day, blood glucose level were measured by glucometer and the rats were sacrificed to collect the liver. Expression of AMPK-α1 were assessed by immunohistochemistry. Data were analyzed by ANAVA and Wilcoxon (α=0,05). The results have been showed that there were significant differences in glucose blood level between negative control with the group were given the extract at dose 90 mg/ kgbw, 360 mg/kgbw and positive control, between dose 180 mg/kgbw with dose 360 mg/kgbw and positive control. A significant differences of expression of AMPK-α1 showed between dose 180 mg/ kgbw with dose 360 mg/kgbw and positive control. The conclusion of this study was the extract ethanol of Singawalang leaves reduce blood glucose level in diabetes mellitus rat models through increase in the expression of AMPK-α1.
Authors and Affiliations
Arifa Mustika, Roostantia Indrawati, Gadis M. Sari
Keywords
Related Articles
- EP ID EP369302
- DOI 10.15416/ijcp.2017.6.1.22
- Views 85
- Downloads 0
Correlation between Low Grade Inflammation with Endothelial Progenitor Cells Surface (EPCs) Marker in Hypertension
Patients with hypertension have been shown to express reduced number and functional capacity of endothelial progenitor cells (EPCs). Recent study reveals that EPCs contain an important capability to maintain endothelial...
Study of the Use of Antibiotics with ATC/DDD System and DU 90% in Digestive Surgery in Hospital in Bandung
Antibiotics are the most widely prescribed medicines in the hospital. Improper use of antibiotics can cause various problems, such as treatment will be more expensive, more toxic side effects, and the emergence of widesp...
Evaluation Management of Drugs and Relations with Quality of Outpatient Pharmacy Services in One of Hospital Pontianak City
Nowadays government policy which embodies the National Social Security System (SJSN) where the presence of this system that every Indonesian people entitled to social security to be able to meet the basic needs of living...
Effectiveness and Toxicity Risk of Intravenous Aminophylline in Exacerbation Asthma Treatment
Asthma is a chronic inflammatory disease in the respiratory tract that has a considerable global prevalence. The worsening of asthma is an asthma attack that causes asthma symptoms to increase and decreased lung function...
Cost of Illness and Cost Containment Analysis Using Empirical Antibiotic Therapy in Sepsis Patients in Bandung
The aims of this study were to analyze cost of illness (COI) and cost containment analysis using empirical antibiotic therapy in sepsis patients with respiratory infection in a hospital in Bandung. A cross sectional meth...