Effect of pH and Temperature on Secondary Metabolite Isolated from Soil Bacteria
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 2
Abstract
Secondary metabolites used to treat infections caused by microbial pathogens. It can cause illness to humans and animals. This study was carried out to screen for potential antimicrobial producing microbes from soil samples collected from different area of Garhwal region in Uttarakhand, India. Dermatophytes are a major group of closely pathogenic fungi that infect skin, hair and nails in humans and animals. In the present study, a trial was done to find out a new antimicrobial agent producing bacteria from soil samples. Antifungal activity of each bacterial isolate against dermatophytic fungus was performed with dual culture and agar well diffusion methods using SDA medium. All the isolated bacterial colonies were observed for primary screening for their anti-dermatophytic activity against the pathogenic species of dermatophytes Trichophyton (MTCC-272), Epidermophyton, (MTCC-465), Microsporum (MTCC-964), Candida albicans, and A. niger were screened by well diffusion methods. Among the total 75 bacterial isolates, only 15 of them were capable of synthesizing antimicrobial metabolites in primary screening. Out of these fifteen isolated bacterial sp. only four Bacterial colonies were found to most potent that was obtained from agricultural region of Srinagar Garhwal, in Uttarakhand found to exhibit the highest antagonistic and anti-dermatophytic activity against most of the used pathogenic dermatophytes in the study. The Physiochemical and biochemical characters of the isolated bacterial species were matched with Bacillus and Pseudomonas sp. Then antifungal activity was measured in different pH and temperature range parameter. Thus, isolated strain was given the suggested name PA-4(a), PA-2(a), PA-2 (PK-1), and PA-1(E). This study indicates that microorganisms isolated from agriculture land of Garhwal region in Uttarakhand (India) soil could be an interesting source of antimicrobial bioactive compound.
Authors and Affiliations
Neelam Bamola, R. K. Gupta
Keywords
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- EP ID EP671181
- DOI 10.21276/ijlssr.2018.4.2.19
- Views 86
- Downloads 0
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