Phenotypic and Molecular Detection of Mycobacterium avium subsp. paratuberculosis in Small Ruminants Clinically Suspected with Johne᾿s Disease
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 5
Abstract
Johne's disease (JD) or Paratuberculosis (PTB) has gained a great attention by many industrial countries for its severing economic losses and possibly zoonotic concerns. In the current study conventional clinical and direct microscopic examination compared to real time polymerase chain reaction (RT-PCR) were used to diagnose JD in clinically suspected small ruminants. Clinical examination revealed 130 (8.7%) suspected cases that showed history of emaciation and diarrhea out of the total examined (1500) animals. Direct microscopy of Ziehl-Neelsen (ZN) stained smears (130) revealed 62 (47.7%) acid fast bacteria resembled Mycobacterium avium subsp. paratuberculosis (MAP). RT-PCR insertion sequence gene (IS900) detected MAP in 25 (65.8%) out of 38 fecal samples harbored acid fast bacilli. We were concluded and recommended that RT-PCR considers the most rapid confirmatory method for screening and diagnosis of the MAP in comparison to low specific conventional phenotypic methods, which still remained valuable techniques in the diagnosis of JD in developing countries.
Authors and Affiliations
Mohamed A. I. Hamid, Galal Eldin E. Mohammed, Amel O. Bakhiet, Elhassan M. A. Saeed
Isolation and Characterization of Pigments from Marine Soil Microorganisms
Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for various applications like dairy, pharmaceutical, and food etc. In this study, three types of p...
Study of the Cytogenetical Effects on Meiotic Chromosomal Abnormalities Induced by Mutagens in
An attempt was made to study the Cytogenetical effects of gamma rays and ethyl methane sulphonate on meiotic chromosomal abnormalities in two cultivars viz., PKV-1 and JS-335. The most frequently observed aberrations in...
Comparison of Antibacterial Efficacy of Aqueous Suspension, Alcoholic Extract and their Combination of Stevia rebaudiana against two Cariogenic Organisms- An in-vitro study
Objectives- The purpose of this study was to compare the effectiveness of aqueous suspension, alcoholic extract and their combination of Stevia rebaudiana against Streptococcus mutans and Lactobacillus acidophilus. Meth...
Effect of pH and Temperature on Secondary Metabolite Isolated from Soil Bacteria
Secondary metabolites used to treat infections caused by microbial pathogens. It can cause illness to humans and animals. This study was carried out to screen for potential antimicrobial producing microbes from soil samp...
Expression Profiling of FasL Gene in Human Blood Tissues and its Correlation with Severe Acute Pancreatitis (SAP)
Blood leukocytes have a vital role in easing general inflammation through acute pancreatitis. Irrespective of topical growth in the considerate to the intricate pathogenesis of pancreatitis and the existence of the disea...