Phenotypic and Molecular Detection of Mycobacterium avium subsp. paratuberculosis in Small Ruminants Clinically Suspected with Johne᾿s Disease
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 5
Abstract
Johne's disease (JD) or Paratuberculosis (PTB) has gained a great attention by many industrial countries for its severing economic losses and possibly zoonotic concerns. In the current study conventional clinical and direct microscopic examination compared to real time polymerase chain reaction (RT-PCR) were used to diagnose JD in clinically suspected small ruminants. Clinical examination revealed 130 (8.7%) suspected cases that showed history of emaciation and diarrhea out of the total examined (1500) animals. Direct microscopy of Ziehl-Neelsen (ZN) stained smears (130) revealed 62 (47.7%) acid fast bacteria resembled Mycobacterium avium subsp. paratuberculosis (MAP). RT-PCR insertion sequence gene (IS900) detected MAP in 25 (65.8%) out of 38 fecal samples harbored acid fast bacilli. We were concluded and recommended that RT-PCR considers the most rapid confirmatory method for screening and diagnosis of the MAP in comparison to low specific conventional phenotypic methods, which still remained valuable techniques in the diagnosis of JD in developing countries.
Authors and Affiliations
Mohamed A. I. Hamid, Galal Eldin E. Mohammed, Amel O. Bakhiet, Elhassan M. A. Saeed
Keywords
Related Articles
- EP ID EP671115
- DOI 10.21276/ijlssr.2018.4.5.9
- Views 88
- Downloads 0
Regeneration of Plantlets from Rhizome Bud Explants of Lasia spinosa (Lour.) Thwaites- A Medicinal Plants of Assam
In numerable medicinal plants are commercially propagated through tissue culture for large production of elite material (Rhizome buds of Lasia spinosa {Lour.}). Thwaites could be induced on Murashige and Skoog (MS) mediu...
Effect of pH and Temperature on Secondary Metabolite Isolated from Soil Bacteria
Secondary metabolites used to treat infections caused by microbial pathogens. It can cause illness to humans and animals. This study was carried out to screen for potential antimicrobial producing microbes from soil samp...
Studies on Population Characteristics of Frigate tuna, Auxis thazard (Lacepede,1800) Occurring in the North West Coast of India
This study is an attempt to understand the population characteristics such as Length-weight relationships, age, growth, mortality, length at age and exploitation rate of Frigate tuna, Auxis thazard occurring in the North...
Gene Frequencies of Haemoglobin Genotype, ABO and Rhesus Blood Groups among Students Population of a Private University in Nigeria-Implications for Blood Banking
Background- ABO and Rhesus blood groups are the two most important blood group systems of clinical significance. ABO is classified into four major groups (A, B, AB and O), while Rhesus is classified as positive or negati...
Comparison of Serum Levels of Tumor Necrosis Factor Alpha (TNF-α) in Batak Male Schizophrenic Patients Versus Healthy Controls
Schizophrenia is a common psychotic disorder, with a risk of about 1%, the etiology of schizophrenia unknown, one of which includes immunological disorders. Although, there are conflicting results, most studies focusing...