EFFECT OF POLY (ADP-RIBOSE) POLYMERASE INHIBITION ON MORPHO-FUNCTIONAL STATE OF IMMUNOCYTES UNDER THE CONDITION OF EXPERIMENTAL ENDOTOXEMIA IN MICE
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2019, Vol 8, Issue 8
Abstract
Recent in vivo and in vitro evidence suggest that inhibitors of poly (ADP-ribose) polymerase (PARP)-1 could be promised in the treatment of inflammatory disorders and conditions associated with an inflammatory component. In our work, we used a murine model of lipopolysaccharide (LPS) induced endotoxemia to investigate the effect of PARP-1 inhibitor, 4-hydroxyquinazoline (4-HQN), on morpho-functional state of the cells of innate and adaptive immunity. We have found that the administration of LPS to mice induced an inflammatory process with significant damage to the immune system cells. There was a pronounced genotoxic stress of immunocytes (according to Comet assay), a loss of their viability what was accompanied by an increased cell death by necrosis and apoptosis, as well as a significant increase in functional and metabolic activity of neutrophils (according to the nitroblue tetrazolium (NBT) test and the lysosomal-cationic test (LCT)). Introduction to the endotoxemic mice of 4-HQN exhibited cytoprotective effect on thymus and lymph node cells: reduced DNA damage and cell death, especially necrosis. 4-HQN administration also contributed to the normalization of neutrophil activity. These findings indicate that pro-inflammatory and immunogenic necrotic cell death and an increase of active oxygen form (AOF) generation caused by PARP-1 may be important mechanisms for enhancing LPS-induced immune inflammation. Consequently, our results indicate that PARP-1 is involved in cell damage, in particular immunocytes, during experimental endotoxemia, as well as indicate the promising use of PARP-1 inhibitors as cytoprotectors in the complex prevention and treatment of endotoxin-induced diseases.
Authors and Affiliations
Nataliya Grushka
Keywords
Related Articles
- EP ID EP668352
- DOI -
- Views 129
- Downloads 0
EFFECT OF OREXIN DRUG AND SOME MINERALS ON RATS EXPOSED TO OBESITY AND ALZHEIMER DISEASE
The affect of administration of some minerals on rats exposed to obesity and Alzheimer disease was studied. Thirty rats of Sprague Dawley (weighting 200±10 g) were divided into sex groups (5 rats). One group considered a...
ANTI - HIV HERBAL DRUGS
Immuno Deficiency Syndrome (AIDS) is a clinical syndrome that is the result of infection with Human Immuno Deficiency (HIV), which causes profound immunosuppression. Current therapies available for symptomatic treatment...
COMPARATIVE IN-VITRO BIOAVAILABILITY STUDIES ON DIFFERENT BRANDS OF BACLOFEN TABLET AVAILABLE ON BANGLADESH MARKET
The presence of numerous generic brands of Baclofen in the local drug market makes the situation difficult for health professionals and patients to choose the appropriate product. This study was intended to evaluate the...
“REVIEW OF MEDICINAL FLOWERS IN INDIA”
Apart from their aesthetic sense, flowers have medicinal and nutritive properties also. An attempt has been made to present the uses of flowers that are mentioned in various Ayurvedic texts. Separate chapters have been m...
KNOWLEDGE, ATTIUIDUE, AND PRACTICE TOWARDS NEEDLE STICK INJURY AMONG NURSING STUDENTS IN KHARTIOUM STATE/SUDAN
Needle stick injuries (NSI) are defined as wounds that are caused by sharp objects like hypodermic needles, due to improper handling or manipulation of needles in different activities such as recapping and failure to dis...