Effect of Population Doublings on Differentiation Capacity of Human Adipose-Derived Stem Cells: Establishing Standard Guidelines for Clinical Translational Applications
Journal Title: International Journal of Stem Cell Research and Transplantation (IJST) - Year 2016, Vol 0, Issue 1
Abstract
Human adipose-derived stem cells (ASCs) are providing promise in clinical soft tissue replacement and repair. This study investigated the effect of multiple population doublings (PD) on ASCs growth rates and cell specific differentiation capacities (DC) to evaluate potential inter-individual specimen growth rates and DC for in vitro cell expansion use in future clinical applications. A decreased cell growth rate and DC were observed in long term cell culture when PD reached to 10.1. Early PD specimens with PD range from 1.6 to 6.2 showed the average population doubling time (PDT) as 5.1 days. There was no significant difference in ASCs growth rates among individual specimens (n=4, p=0.184). The DC over PD range 1.6 to 6.2 also showed no significant changes (osteogenic, n=3, p=0.472; chondrogenic, n=3, p=0.878; adipogenic, n=3, p=0.256). Our preliminary data showed the importance of PD for in vitro ASCs expansion. The cell growth rate and DC of ASCs were relatively stable up to PD 6.2. Beyond this PD, there was a trend of decreased growth rate and DC. Therefore, our study suggested that future in vitro expansion of ASCs for clinical application should consider limiting expansion of ASCs to PD 6.2 or less to ensure the cell quality. Use of specific PD instead of the non-specific passage numbers would be a better standardization of in vitro ASCs expansion for their clinical translational applications.
Authors and Affiliations
Spencer A. Brown
Keywords
Related Articles
- EP ID EP198182
- DOI 10.19070/2328-3548-SI02001
- Views 81
- Downloads 0
Cancer Stem- Like Cells in Melanoma Progression, Resistance and Recurrence: Significance for Melanoma Treatment
Human malignant melanoma is a highly aggressive tumor which demonstrates heterogeneity and a propensity to drug resistance. Despite improved treatment options, patients with advanced malignant melanoma continue have a po...
Cataract How Important Is Age of Intervention?
Purpose: To study effect of age of intervention on visual outcome following treatment of pediatric patients with cataract. Setting: Tertiary eye care centre in Dahod at the trijunction of Gujarat, Madhya Pradesh, and Raj...
Neonatal Eye Defects and Stem Cell Therapeutics
Present review aims to describe various neonatal eye defects and its stem cell therapeutics. This article attempts to highlight common causes of visual impairment in infants and present the recent approaches used for res...
Differentiation of Neural Progenitor Cells from Pluripotent Stem Cells in Artificial Niches
Neural progenitor cells (NPCs) derived from pluripotent stem cells (PSCs) provide a promising cell source for disease modeling, drug screening, and tissue engineering. To fulfill the potentials of PSC-derived NPCs, a ser...
Generation and characterization of induced pluripotent stem cells in domestic Asian water buffalo (Bubalus bubalis)
Induced pluripotent stem cells (iPSCs) have numerous applications in livestock to improve production traits, disease resistance, biopharming, conservation of germplasm, disease modeling, regenerative medicine etc. Here,...