Effect of red blood cell preservation solution on serum agglutination reaction of specific patients
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 11
Abstract
Objective To explore the precautions of pre-transfusion examination in patients with antibodies to erythrocyte protective solution, discrepant ABO blood typing results, and positive unexpected antibodies, so as to ensure the safety of blood transfusion. Methods The screen cells were divided into two groups according to the presence or absence of washing reagent red blood cells in normal saline. One group had untreated forward typing cells, antibody screening cells and identification panel, and the other group had saline-washed reverse typing cells, antibody screening cells and identification panel. The experiments were carried out by microcolumn gel method, saline medium method and polyamine method to analyze the effect of red blood cell preservation solution on serum agglutination reaction of specific patients. Results Among the 8 patients, forward typing was AB (+ ) in 1 patient, B (+ ) in 4, and A(+ ) in 3, and the reverse typing were interfered. The plasma of 8 patients agglutinated with unwashed reverse typing cells (saline tube method), screen cells and identification panels (saline tube method plus cassette method), while not agglutinated with the polybrene method. The interference was eliminated as using washed reverse typing cells (salinetube method), screen cells and identification panels (saline tube method plus cassette method). Conclusion The erythrocyte preservation solution affected patients’ blood group typing, but not affected the outcome of blood transfusion as no adverse reactions occurred.
Authors and Affiliations
Xiao HAO, Lining MA, Huanan LIU, Xiaoye XIA, Xueqin DIAO, Xuejing LI
Keywords
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- EP ID EP738416
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.11.021
- Views 56
- Downloads 0
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