Effect of RNA interference on WD101 gene of Schistosoma japonicum
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 2
Abstract
Objective: To study the effect of RNA interference (RNAi) on WD101 gene and its effect on the expression of WD101 mRNA and protein in Schistosoma japonicum. Methods: Doublestranded RNA (dsRNA) WD101 gene and control gene (lacZ) were generated by in vitro transcription and transfected into mechanically transformed schistosomula. The total RNA and protein were isolated simultaneously using TRIzol reagent. The expression levels of mRNA and the protein were determined by quantitative real-time PCR (qPCR) and Western blotting, respectively. After injected dsRNA-electroporated schistosomula into BALB/c mouse six weeks, the male and female reproductive organs were observed and measured under the confocal laser scanning microscope. Results: After 1, 3 and 5 d of RNAi, WD101 mRNA level was decreased by 15%, 39%, and 58% in experiment group compared to that in control group; meanwhile, WD101 protein level was decreased by 11%, 28%, and 43% in experiment group compared to that in control group. There were significantly more sperms in testicular lobes in experiment group than that in control group, while there were no significant differences in terms of ovary and vitelline glands between two groups. Conclusions: The dsWD101-RNAi can effectively induce suppression of WD101 gene expression at both mRNA and protein levels. WD101 gene might be a reproduction-related gene in Schistosoma japonicum.
Authors and Affiliations
Peng Zhang
Keywords
Related Articles
- EP ID EP268114
- DOI 10.4103/1995-7645.225021
- Views 97
- Downloads 0
GC–MS analysis of volatile compounds of Perilla frutescens Britton var. Japonica accessions: Morphological and seasonal variability
Objective: To investigate the composition of volatile compounds in the different accessions of Perilla frutescens (P. frutescens) collected from various habitats of China and Japan. Methods: In the present study, the ess...
Electrophysiological changes of autonomic cells in left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with chronic heart failure
Objective: To investigate the electrophysiological changes of autonomic cells in left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with chronic heart failure. Methods: Guinea pigs mode...
Prediction of promiscuous T cell epitopes in RNA dependent RNA polymerase of Chikungunya virus
Objective: To explore RNA dependent RNA polymerase of Chikungunya virus (CHIKV) and develop T cell based epitopes with high antigenicity and good binding affinity for the human leukocyte antigen (HLA) classes as targets...
A study on the optimization and characteristics of enzymatic preparation of Artocarpus heterophyllus Lam (Jackfruit) seed resistant starch
Objective: With the development of the Artocarpus heterophyllus Lam (A. heterophyllus) processing industry, A. heterophyllus Lam seed as by-products, usually are cooked to eat or discarded, and no industrial application...
An updated systematic review of Zika virus-linked complications
Objective: To determine the breadth of Zika virus (ZIKV)-associated brain anomalies in neonates and adults. Methods: Systematic review was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-...