Effect of Sex Hormones and Prolactin on Sickle Cell Erythrocyte Polymerisation in Portharcourt, Rivers State, Nigeria
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 1
Abstract
Sickle cell disease (SCD) is an inherited hematological disorder that causes red blood cells to break down continuously. This leads to a rigid, sickle like shape under certain conditions, causing polymerization of the sickled hemoglobin. This study was undertaken to know whether sex hormones (estradiol, progesterone, testosterone and prolactin) exert any effect on the polymerization of sickle cell erythrocytes in vitro and the possibility of these hormones having an effect on the sickling phenomenon. The hemoglobin polymerization test was carried out when hemoglobin S undergoes gelation after it was deprived of oxygen using 2% sodium metabisulphite as reductant. The polymerization inhibition studies were shown that estrogen, progesterone, testosterone and not prolactin had a statistical significant reduction effect (P<0.05) on the polymerization of the sickle cell erythrocytes. The polymerization of the sickle cell erythrocytes was reduced to 50.90%, 62.74%, 67.56%, and 92.16% at the concentration of 50.0 pg/ml of estrogen, 5.0 ng/ml of progesterone, 6.0 ng/ml of testosterone, and 7.0 ng/ml of prolactin in the same order. This effect was achieved at a low concentration of these hormones. Higher concentrations of the hormones increased polymerization. The result suggests that using the hormones substances at low concentrations can help to ameliorate the intracellular polymerization of sickle cell hemoglobin.
Authors and Affiliations
Ezeiruaku FC, Eze EM, Ukaji DC
Keywords
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- EP ID EP670631
- DOI 10.21276/ijlssr.2018.4.1.17
- Views 90
- Downloads 0
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