EFFECT OF SODIUM NITRITE EXPOSURE ON THE IMMUNE RESPONSES AGAINST OF RIFT VALLEY FEVER VACCINE IN MICE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 7
Abstract
Objective: Daily exposure to food preservatives constitutes a major crisis to children especially during vaccination so; the aim of the study is to assess the effect of sodium nitrite on the immune responses against Rift Valley fever vaccine (RVFV) in Swiss mice. Methods: Mice were divided into four equal groups: group 1 (control) was orally administrated with distilled water (2 ml/kg b. wt.); group 2 was orally administrated with the acceptable daily intake (ADI) of sodium nitrite 0.07 mg/kg b. wt. daily for 21 d, group 3 was vaccinated with inactivated RVFV two times and group 4 was orally given sodium nitrite and vaccinated as group 3. Blood samples were collected from all groups two weeks after booster vaccination. The leucocytic indices and the neutrophil-lymphocyte ratio (NLR) were determined to assess the cell-mediated immunity. The humoral immunity was evaluated using direct enzyme-linked immune-sorbent assay (ELISA) test and serum neutralization test (SNT). Results: Sodium nitrite significantly increased the neutrophil index and the NLR whereas; it decreased the total leucocytic count and lymphocyte index in both non-vaccinated and vaccinated mice. Moreover, sodium nitrite significantly decreased both the IgG titer and the efficiency of vaccination through increasing the ED50 value. Conclusion: Sodium nitrite existed an immune-suppressive effect on both cellular and humoral immune responses in mice.
Authors and Affiliations
Khaled Abo-EL-Sooud, Mohamed Hashem, Yasmina ABD ELHAKIM, Gehan kamel, Mona ELEIWA, Ali Gab-Alllah
Keywords
Related Articles
- EP ID EP593430
- DOI 10.22159/ijpps.2019v11i7.33443
- Views 57
- Downloads 0
ISOLATION OF ANGIOTENSIN-CONVERTING ENZYME INHIBITOR PRODUCING BACTERIA FROM COW MILK
Objective: To evaluate the potential of protease producing organism for the production of Angiotensin I–converting enzyme (ACE) inhibitor by fermentation of various protein substrates.Methods: Bacterial strains were is...
FORMULATION AND EVALUATION OF ETODOLAC LECITHIN ORGANOGEL TRANSDERMAL DELIVERY SYSTEMS
Objective: Etodolac (ETD) is a non-steroidal anti-inflammatory drug used for the acute and chronic treatment of rheumatoid arthritis. It exhibits poor water solubility so its bioavailability is limited. Long term use of...
ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS OF JUSTICIA SPICIGERA ETHYL ACETATE FRACTION AND CHARACTERIZATION OF ITS ANTHOCYANIN CONTENT
Objective: The antioxidant and hepatoprotective activities of ethyl acetate (EA) fraction of the dried aerial part of Justicia spicigera were evaluated and the characterization of its anthocyanin content was done. Metho...
ENTADA PHASEOLOIDES ATTENUATES SCOPOLAMINE INDUCED MEMORY IMPAIRMENT, NEURO-INFLAMMATION AND NEURO-DEGENERATION VIA BDNF/TRKB/NFΚB P65 PATHWAY IN RADIAL ARM MAZE
Objective: The present study investigates the protective effects of crude extract from seeds of Entada phaseoloides and isolated compounds in enhancing cognition in Scopolamine induced learning and memory impairments in...
SPECTROPHOTOMETRIC DETERMINATION OF OXYBUTININE HYDROCHLORIDE BY ION-PAIR EXTRACTIONIN IN PHARMACEUTICAL PREPARATIONS
Objective: Simple, sensitive, precise, reproducible and validated visible spectrophotometric methods have been developed for the determination of an antimuscarinic drug, namely oxybutynin hydrochloride (OXB) in pure form...