EFFECT OF SODIUM NITROPRUSSIDE ON ACTIVITIES OF ANTIOXIDANT AND GLUTATHIONE-RELATED ENZYMES IN LEAVES OF MAIZE SEEDLINGS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
Sodium nitroprusside was used as nitric oxide (•NO) donor to investigate effects of •NO on the activity of antioxidant enzymes in leaves of maize seedlings. Seeds of maize hybrid Kharkivskyj 195 MB (Zea mays L.) were used for the experiments. Leaves of 10-day old seedlings were cut off and transferred into round-bottomed flasks with distilled water or 0.1, 0.5 and 1.0 mM sodium nitroprusside solution (SNP). They were incubated for 24 h at constant shaking at 150 rpm and illumination with an 18 W fluorescent light, giving 800 lux of light intensity (16/8 h day/night regime and 26°C). The equimolar concentrations of potassium hexacyanoferrate (II) (PCF), were used as an additional control to SNP, since it has a chemical structure similar to SNP but lacks the ability to produce •NO. It was shown that treatment of leaves with SNP did not affect catalase activity, but PCF exposure enhanced it by 38-49%. The activity of ascorbate peroxidase was increased by 44-60% in leaves treated with all three concentrations of PCF, while only 0.1 and 0.5 mM of SNP enhanced it by ~60%. Guaiacol peroxidase activity was substantially suppressed by 71-80% and 45-69% in leaves incubated with both effectors, PCF and SNP, respectively. Potassium hexacyanoferrate (II) at all concentrations used did not affect glutathione-S-transferase activity in the leaves of maize seedlings, while 0.5 and 1.0 mM SNP increased it by 18 and 28%, respectively. Glutathione reductase activity was higher in leaves treated with 0.1 and 1.0 mM PCF by 16 and 39%, respectively, whereas 0.5 mM PCF did not change it. At the same time, SNP at all concentrations used increased GR activity by 40-60%. The increase of enzyme activity might result from mild nitrosative/oxidative stress induced by •NO donor, and/or to some extent by iron and cyanide ions, which released at SNP decomposition.
Authors and Affiliations
Y. V. VASYLYK, N. M. SEMCHUK, OK. V. LUSHCHAK
Keywords
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