EFFECT OF SOLVENT EXTRACTION SYSTEM ON THE ANTIOXIDANT ACTIVITIES OF ALGAE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: The present study aimed to determine the antioxidant activities of five algae including Oedogonium globossum, Pithophora oedogonia, Rhizoclonium hieroglyphicum, Scytonema ocellatum and Spirogyra communis and the effect of different solvent (benzene, chloroform, acetone and methanol) used for the extraction of active components from algae.Methods: The five algae were extracted with four different solvents e. g benzene, chloroform, acetone and methanol. The Folin-Ciocalteu procedure was used to estimate the amount of total phenolic content in different extracts of algae. Total flavonoid content was estimated by aluminium chloride colorimetric method. The DPPH radical scavenging assay was used to find out the antioxidant activities of algae under investigation.Results: The total phenolic content varied from 9.80±0.29 to 44.44±2.26 mg/g, 7.26±0.08 to 21.30±0.17 mg/g, 9.82±0.12 to 50.14±0.99 mg/g and 11.51±0.17 to 54.02±0.58 mg/g dry material in the benzene, chloroform, acetone and methanol extracts of the algae respectively. The flavonoid content was found to be the highest in the acetone extract of Scytonema ocellatum (593.81±2.69 mg/g) and flavonol content was found to be the highest in the chloroform extract of Scytonema ocellatum (448.22 ± 4.36 mg/g) whereas least amount of flavonoid present in the benzene extract of Oedogonium globossum 8.91±0.37 and least amount of flavonol was present in the chloroform extract of Spirogyra communis (13.54±1.07 mg/g). The highest DPPH radical scavenging was observed in the methanol extract of Scytonema ocellatum with IC50 = 0.24±0.003 mg dry material. The reducing power of the extracts of the algae was also evaluated as mg AAE (ascorbic acid equivalent)/g dry material. Chloroform extract of Rhizoclonium hieroglyphicum showed the highest reducing power 185.56±2.00 mg AAE/g dry materials.Conclusion: Thus it could be suggested that these algae can be utilized as natural antioxidant.
Authors and Affiliations
Tapan Seal, Nilu Halder, Kausik Chaudhuri, Sankar Narayan Sinha
Keywords
Related Articles
- EP ID EP579440
- DOI -
- Views 75
- Downloads 0
IMMUNOMODULATORY ACTIVITY OF AN ACETONE EXTRACT OF TERMINALIA BELLERICA ROXB FRUIT ON THE MOUSE IMMUNE RESPONSE IN VITRO
Objectives: To investigate the immunomodulatory activity of an acetone extract of T. bellerica fruit.Methods: Mitogen induced-lymphocyte proliferation using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium b...
BARLERIA MONTANA WIGHT AND NEES–A PROMISING NATURAL ANTI-INFLAMMATORY AGENT AGAINST FORMALIN INDUCED INFLAMMATION
Objective: To evaluate the anti-inflammatory activity of the ethanolic leaf extract of Barleria montana (B. montana) against formalin induced inflammation.Methods: Male albino wistar rats were pretreated with oral doses...
PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT STUDIES OF LEAF AND STEM BARK EXTRACTS OF POLYALTHIA FRAGRANS (DALZ.) BEDD.–AN ENDEMIC SPECIES OF WESTERN GHATS
Objective: Phytochemical constituents of various solvent extracts of leaf and stem bark of Polyalthia fragrans (Dalz.) Bedd were screened. Antimicrobial and antioxidant activities of the extracts were evaluated.Methods:...
ESTIMATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF THEVETIA PERUVIANA (PERS.) K. SCHUM.
Objective: The aim of this investigation was to estimate antioxidant and antibacterial activity of different crude extracts of Thevetia peruviana (Pers.) K. Schum.Methods: Aqueous and alcoholic crude extracts of differen...
AN IMPLICATION OF ACTINOMYCETES ON HUMAN WELL-BEING: A REVIEW
This review conceptualizes about the actinomycetes and its contribution to human health by playing a key role as bioactive secondary metabolites, such as enzymes, antibiotics and pigments, leading to their diverse applic...