Effect of taurine administration on activity of superoxidedismutase in rat tissues
Journal Title: Біологія тварин - Year 2015, Vol 17, Issue 3
Abstract
The aim of work was to study the activity of superoxide dismutase and its isozymes in rat tissues under the conditions of prolonged oral administration of taurine. Male Wistar rats weighing 190–220 g and age 4.5 months, were divided into three groups. Rats for 28 days, daily, though esophagus probe were injected: control — drinking water and two experimantal — taurine solution of 40 (I experimental group) and 100 (II experimental group) mg/kg, respectively. After completing the research animals were decapitated under light chloroform anesthesia and liver, brain, testis and thigh muscle tissues were isolated. Tissues were homogenized, centrifuged. In supernatant was determined total superoxide dismutase and isozyme content after electrophoresis in 10 % polyacrylamide gels, staining with tetrazolium nitroblue. Given the percentage isozyme, the activity of each was deducted from the total activity of the enzyme. It was found that activity of brain superoxide dismutase remains within control under the conditions of prolonged per oral administration of taurine. However, cytoplasmic redistribution of isozyme activities occurs — S2 increases, and S3 and S1 decreases. The superoxide dismutase liver of I experimental group animals is the highest. This occurs because of the increase in activity of extracellular (S5) and cytoplasmic (S2) isozymes. With the increase of SOD activity in testes of II experimental group increases the quantity and value of all isozymes. In muscles of both experimental groups increased total activity, with it grow extracellular (S5) and cytoplasmic (S1) activity of superoxide dismutase isozymes. Consequently, the impact of taurine long-term oral administration on activity of superoxide dismutase is realized through isozyme content change, and is tissue- and dosespecific.
Authors and Affiliations
R. D. Ostapiv, V. V. Manko
Keywords
Related Articles
- EP ID EP345602
- DOI -
- Views 107
- Downloads 0
Influence of aflatoxin В1 on prooxidant-antioxidant balance in cells of white rats
The article presents the results of the researches of influence of aflatoxin В1 (AFB1) on activity of enzymes of the antioxidant system (glutathione peroxidase, glutathione reductase and glutathione-S-transferase) and co...
ShRNA-mediated knockdown of pituitary tumor transforming gene 1 (PTTG1): optimization of the effect on early osteoblast differentiation
Rheumatoid arthritis (RA) is a chronic inflammatory disorder characterized by massive joint destruction. Bone erosion belongs to the most painful consequences of RA that are tightly associated with disease severity and p...
Blood serum biochemical indices of different categories of pigs by qualitative composition of muscle tissue
The article presents the results of studies of biochemical parameters of blood serum in young pigs taking into account their distribution into categories according to physico-chemical properties and chemical composition...
Perspectives of studying causes and consequences of the mass death of honeybees
This review is devoted to the problem of mass death of bees, also known as “colony collapse syndrome”. The available data on the possible causes of this phenomenon has been summarized. Special attention is given to the a...
The comparison of qualitative composition of the muscle tissue of brown trout, rainbow trout and brook trout
Intensification and development potential of the aquaculture sector have created problems relating to the ecological purity and quality of the final fish product. In the conditions of Ukraine, there is extremely little i...