Effect of Taurine on the antimicrobial efficiency of Gentamicin
Journal Title: Advanced Pharmaceutical Bulletin - Year 2011, Vol 1, Issue 2
Abstract
Context: Gentamicin is mainly used in severe infections caused by gram-negatives. However toxicity including nephrotoxicity and ototoxicity is one of the most important complications of its treatment. The production of free radicals seems to be involved in gentamicin toxicity mechanism. Taurine, a major intracellular free β-amino acid, is known to be an endogenous antioxidant. So potentially the co-therapy of taurine and gentamicin would reduce the adverse effects of the antibiotic. Objectives: In this study, we wished to know the effect of taurine on the antibiotic capacity of gentamicin. Methods: strainsof P. aeruginosa, E. coli, S. aureus and S. epidermidis were used as test organisms. Minimum inhibitory concentrations of gentamicin in the presence and absence of taurine at quantities from 40 to 2 mg/L were determined using macro-dilution method. Results: MICs were determined in the various concentrations of taurine for bacterial indicators. The MIC values of gentamicin for P. aeruginosa, S. aureus and E. coli remained unchanged in the values of 2.5, 5 and 20 μg/ml respectively in the absence and presences of different concentrations of taurine. The bactericidal activity of gentamicin against S. epidermidis was increased by addition of taurine in the concentrations higher than 6 mg/L. Conclusion: According to our study the antibacterial activity of gentamicin against the indicator microorganisms were not interfere with taurine at selected concentrations. Further in vivo studies are needed to establish if a combination of gentamicin and taurine would have the same effect.
Authors and Affiliations
Mina Islambulchilar, Mohammad Reza Sattari, Mohammad Sardashti, Farzaneh Lotfipour
Keywords
Related Articles
- EP ID EP87112
- DOI 10.5681/apb.2011.010
- Views 136
- Downloads 0
Skin Infection Management Using Novel Antibacterial Agents
Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) cause difficulties in the management of skin and soft tissue infections and have led to morbidity and mortality in hospital-acquired infection...
Polymer Percolation Threshold in Multi-Component HPMC Matrices Tablets
Introduction:The percolation theory studies the critical points or percolation thresholds of the system, where one component of the system undergoes a geometrical phase transition, starting to connect the whole system.Th...
Thermoanalytical Investigation of Terazosin Hydrochloride
Purpose: Thermal analysis (TGA, DTG and DTA) and differential scanning calorimetry (DSC) have been used to study the thermal behavior of terazosin hydrochloride (TER). Methods: Thermogravimetric analysis (TGA/DTG), diffe...
High Performance Liquid Chromatographic Analysis of Almotriptan Malate in Bulk and Tablets
Purpose: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. Methods: Chromatographic separation of ATM was achieved by using a Thermo Scientifi...
Derivative Spectrophotometric Method for Estimation of Antiretroviral Drugs in Fixed Dose Combinations
Purpose: Lamivudine is cytosine and zidovudine is cytidine and is used as an antiretroviral agents. Both drugs are available in tablet dosage forms with a dose of 150 mg for LAM and 300 mg ZID respectively. Method: The m...