Effect of the Pyridoxine Supplements on the Minimum Inhibitory Concentration of the Co-Ampicillin-Cloxacillin against Staphylococcus Aureus: In Vitro Study
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 4
Abstract
Background: Pyridoxine or vitamin B6is a water-soluble vitamin that is naturally present in many foods, added to others, and available as a dietary supplement. Microorganisms synthesized pyridoxine and played a role in their virulence. Objectives: This study aims to evaluate the effect of synthetic (exogenous) pyridoxine against Co-ampiclox susceptible and resistant Staphylococcus aureus. Materials and Methods: This study was done in the Department of Microbiology, College of Medicine, AlMustansiriya University in Baghdad, Iraq from November 2014 to January 2015. A total number of thirty Staphylococcus aureus isolated from infected wounds were obtained from the Laboratories of Al-Yarmouk Teaching Hospital. The antibacterial effect of Co-ampiclox (25µg/ml), pyridoxine (at serial concentrations 1-64µg/ml) or a combination were examined in vitro using broth dilution technique. Results: Four out of thirty isolates were resistant to 25 µg/ml ampiclox. Pyridoxine per se at low concentration (1µg/ml) inhibits the growth of the susceptible isolates by 18.7% while at higher concentrations failed to exert any antimicrobial effect and it reduced the antimicrobial effects of Coampiclox against the susceptible Staphylococcus aureus. Pyridoxine at low concentration (1µg/ml) inhibits the growth of the resistant isolates by 13%. while its combination with Co-ampiclox at concentration 4µg/ml inhibit the mean growth of bacteria by 18.3%. Conclusions: Exogenous pyridoxine exerts antibacterial effect as demonstrated in this study while the endogenous pyridoxine that synthesized by the bacteria is necessary for their resistance and this indicated that pyridoxine exerts dual effect against the growth of the microorganisms
Authors and Affiliations
Batool Hazim Al-Daherie
Keywords
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