Effect of trophic, osmotically active and solidifying components of nutrient medium on the direct embryognesis in spring barley anther culture in vitro
Journal Title: Фактори експериментальної еволюції організмів - Year 2018, Vol 22, Issue
Abstract
Aim. Ambryoidogenesis, or somatic embryogenesis, is known to be the most efficient mode of plant regeneration in plant cell, tissue and organ culture. The investigation was aimed to elucidate effects of trophic and osmogenic components of inductive medium on the frequency of direct embryoidogenesis in spring barley anther culture in vitro and to determine mechanism of morphogenesis improvement coursed by chemically modified starch D-5aM used as a gelling agent instead of agar. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose or mannitol (0.3 M) and solidified with agar or chemically modified starch. Results. A positive effect of combination of high maltose content and chemically modified starch on the induction and regeneration processes in spring barley anther culture in vitro was confirmed. It was also shown that mannitol didn’t keep any growth or development processes going in barley anther and embryo culture, but at the same time this substance had no toxic effect. Conclusions. In order to achieve a high frequency of induction in spring barley anther culture, it is necessary to use medium containing maltose – a low weight component both with trophic and with osmotic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, maltose, embryo formation, plant regeneration.
Authors and Affiliations
О. В. Білинська, П. Г. Дульнєв
Keywords
Related Articles
- EP ID EP618759
- DOI -
- Views 41
- Downloads 0
THE PRODUCTIVITY OF WINTER WHEAT STEMS UNDER DIFFERENT MINERAL NUTRITION
Aim. The aim of this work was to study the influence of mineral nutrition on winter wheat stems productivity. Methods. Winter wheat (Triticum aestivum L.) cultivars ‘Mironivska 808’ and ‘Favoritka’ were grown on mixture...
Academician Andriy Opanasovich Sapegin (to birthday 135 anniversary)
The main biographical data on the life and creative path of Academician AO Sapevin – an outstanding scientist, talented teacher and organizer of science, the pioneer of the leading areas of genetics and selection.Keyword...
Evaluation of toxicity and genotoxicity of nano nanoparticles Ag2S, synthesised by biological matrix, on Drosophila melanogaster Mg. (Diptera: Drosophilidae)
Aim. To evaluate the toxicity and genotoxicity of Ag2S quantum dots that had been synthesized by the mycelium of the fungus Pleurotus ostreatus on Drosophila melanogaster. Methods. The toxicity of nanoparticles was deter...
Качество зерна у линий мягкой пшеницы с генетическим материалом Triticum kiharae
Цель. Triticum kiharae (AtAtGGDD, 2n=42) представляет интерес для улучшения сортов мягкой пшеницы как источник высокого содержания белка, клейковины в зерне и устойчивости ко многим заболеваниям. С целью обогащения геноф...
Вплив трофічних, осмотично активних та гелеутворювальних компонентів живильного середовища на прямий ембріоїдогенез у культурі пиляків in vitro ярого ячменю
Мета. Ембріоїдогенез, або соматичний ембріогенез, є найбільш ефективним шляхом регенерації рослин у культурі in vitro клітин, тканин та органів. Мета досліджень полягала у визначенні впливу на частоту прямого ембріоїдоге...