EFFECT ON IMMUNE RESPONSE AGAINST Pasteurella multocida CAPSULAR TYPE A:1 AND A:4 ON SUPPLEMENTATION WITH PROTEIN PURIFIED DERIVATIVES OF Mycobacterium bovis IN CHICKEN
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
The adjuvant potential of tuberculo-protein of Mycobacterium bovis and Montanide ISA-206 adjuvants in different combination with Pasteurella multocida serotype A:1 and A:4 whole cell antigens have been evaluated in chicken model. Four different groups of chicken were immunized with different combination of adjuvants and humoral immune response was assessed by indirect ELISA. The immune response study revealed that chicken immunized with whole cell antigen in combination with Protein precipitated derivative (PPD) as well as montanide ISA-206 elicited a robust humoral immune response. To assess the protective ability two groups of chicken immunized with P.multocida A:1 were challenged with 1 MLD of Pasteurella multocida A:1; whereas two other groups of chicken immunized with P.multocida A:4 bacterin were challenged with 100 MLD of P.multocida A:4. Challenge studies indicated that both the groups of chicken immunized with P. multocida A:1 whole cell antigen adjuvanted with both Montanide ISA-206 and Mycobacterium bovis PPD as well as only Montanide ISA-206 conferred 100 % protection against P multocida A:1. However two other groups immunized with P.multocida A:4 bacterins could not sustain 100 MLD dose of direct challenge and there were 100% death of all the birds in both the immunized group, but the group immunized with both Montanide and PPD showed delayed death.
Authors and Affiliations
Stanzin Zadon, Vinod Kumar Chaturvedi, Bablu Kumar, Praveen Kumar Gupta, Mayank Rawat, Varsha Paladan
Keywords
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- EP ID EP427747
- DOI 10.18006/2017.5(6).780.785
- Views 50
- Downloads 0
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