Effectiveness of different solutions in decontamination of gutta-percha cones<br /> exposed to Enterococcus faecalis
Journal Title: RSBO - Year 2005, Vol 2, Issue 2
Abstract
The cones of gutta-percha are the main root canal filling materials.The aim of this study was to evaluate the effectiveness of some products in the decontamination of these cones. Eighty (80) cones of gutta-percha #40, separated in 8 groups with 10 cones each one, have been used as sampling. Seventy cones have been contaminated with Enterecoccus faecalis for immersion in saline solution contends 105 to 108 bacterial cells/mL.The groups have suffered decontamination for G1: alcohol 70%; G2: alcohol 70% + iodine 1%; G3: alcohol 70% + chlorexidine 4%; G4: chlorexidine 4%; G5: NaOCl 2,5%; G6: NaOCl 5,25%; G7: saline solution; G8: it was not contaminated and it did not suffer decontamination (control). After 1 minute in contact with each product, five (5) cones have been removed,washed in sterile saline solution and introduced individually in tubes containing BHI solution. The other cones have been removed after 5 minutes in contact with the solutions, repeating the same process of the previous ones. The set of tubes have been agitated per 1 minute and taken to the sterilizer and kept there at 37ºC for 48 hours. The tubes that presented turbidity of BHI solution have been considered positive. It could be observed that in the time of 1 minute there was a bacterial growth in groups 1, 2, 5 and 7 and in the time of 5 minutes only groups 5 and 7 had presented bacterial growth. Group 8 presented one contaminated cone.With the used methodology, we concluded that the alcohol 70% + chlorexidine 4%, chlorexidine 4% and NaOCl 5,25% solutions did not allow the development of E. faecalis, promoting therefore good disinfection of the gutta-percha cones in an adjusted time for clinic practice.
Authors and Affiliations
Flávia FAGUNDES, Denise LEONARDI, Gisele HARAGUSHIKU, Flares BARATTO FILHO, Luiz TOMAZINHO, Paulo TOMAZINHO
Keywords
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