Effectiveness of incipient caries induction in microaerophilic and/or aerobic conditions using Streptococcus mutans and Lactobacillus acidophilus. In vitro studies.
Journal Title: Journal of Stomatology (Czasopismo Stomatologiczne) - Year 2012, Vol 65, Issue 3
Abstract
Introduction. So far various chemical and microbiological models have been used to induce in vitro caries. Demineralizing agents (e.g. acids) caused only surface demineralization. Enamel demineralization at early stage of in vivo caries process has subsurface character. Thanks to microbiological models, using single or poly-bacterial cultures, various environments and different process durations, the development of caries which resemble in vivo stains is possible. Aim of the study. Comparison of effectiveness in in vitro obtainment of incipient caries, using Streptococcus mutans and Lactobacillus acidophilus incubated in BHI and PP media supplemented with 5% sucrose under microaerophilic and/or aerobic conditions. Materials and methods. Experiments were performed on 50 intact teeth coated with human saliva. 25 teeth were incubated in BHI with sucrose and S. mutans (1 month), then with L. acidophilus (3 months) under aerobic conditions at 37°C (Method I). Remaining 25 teeth were incubated in PP with sucrose in the presence of S. mutans in microaerophilic environment (1 month) and then with L. acidophilus under aerobic conditions (3 months) at 37°C (Method II). The enamel lesions were evaluated using visual, probing, SEM and microtomography inspections after 2, 4, 6, 8, 10, 12, 14 and 16 weeks of incubation, respectively. Results. In both methods caries incipiens were obtained. However, in Method II (PP with sucrose, variable conditions of the incubation - microaerophilic and aerobic) caries lesions occurred earlier, were more distinct and corresponded to those observed in vivo. Conclusion. Microbiological Method II, using S. mutans and L. acidophilus, proved more efficient in caries-like enamel lesions induction.<br/><br/>
Authors and Affiliations
Alina Gębska, Anna Kędzia, Barbara Kochańska, Adam Wąsek, Ewa Kwapisz, Peter Bottenberg
Keywords
Related Articles
- EP ID EP57639
- DOI 10.5604/00114553.1001263
- Views 72
- Downloads 0
Variations in orthodontic bracket retention related to base design. Comparison of foil mesh and anchor pylons brackets
of metal brackets with foil mesh (3M, Victory Series), one piece metal brackets (Cannon Ultra) and aesthetic plastic brackets (Cannon Ultra) and to evaluate the sandblasting effect on previously used metal bracket bases...
Results of traumatic injuries of milk teeth and the alveolar process – case study
Injuries to the teeth and nearby tissues are the most frequent cause of children’s presentation at the dental surgery. Complications after injury to milk teeth, the alveolar process of the maxilla and the alveolar part o...
Lichen planus coexisting with diabetes mellitus and hypertension (Grinspan’s syndrome) - cases description
Introduction: Lichen planus is a chronic skin and oral mucosa disease. Coexistence of the oral form of lichen planus with diabetes and hypertension was described for the first time by Grinspan in 1963. Study goal: Two c...
In Vitro Efficacy of Garlic Extract against Candida albicans Biofilms from Children with Early Childhood Caries
Introduction: Garlic (Allium sativum) is known to be a natural antifungal agent with the ability to penetrate cellular and organelle membranes, resulting in cell death of Candida albicans. Aim of the study: The aim o...
The laser method and its effect on the oral mucous membrane in the context of the preprosthetic surgery
Background: Various treatment methods are applied in the preprosthetic surgery. The most frequently used are lasers, standard excision of the lesion with a scalpel and with an electrocauter. As a subject of the analysis...