Effectof differentPre sowing treatments on seed germination of endangered medicinal plant Caesalpiniabonducella (L) Roxb.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 3
Abstract
Caesalpiniabonducella(L.)Roxb.is an important Indian ethno medicinal plant belongs to the family Caesalpiniaceae. All parts of the plant including root, stem, bark, leaves, flower, pod, seed coat and kernel are used in many Ayurvedic preparations. The plant requires seven years for germination in nature due to hard seed coat. Hence this study deals with different treatments like mechanical scarification ,acid scarification, impact of effector substances like KNO3, NaCl,CdSO4, PEG and GA3 on seed germination of nicker nut. Mechanically scarified seeds showed 40% germination. Acid scarification was carried out with con.HCl, con.H2SO4 and HNO3. The treatments were given for 30 minutes, 60 minutes and 90 minutes in each acid. Con.H2SO4 for 60 minutes resulted in 80 % seed germination followed by 10%in Con. HNO3 and 20% in Con. HCl. GA 3 50 ppm and 0.2%KNO3 were also used and the germination rate were 40% and 10% respectively. Germination rate was 30% in 50mM NaCl. PEG and CdSO4 were unsuitable for germination. In vitro seed germination was also studied with different phytohormones and agar. MS medium supplemented with 1mg/l NAA and 0.6 mg/l GA 3 resulted in 80% germination. When the concentration of the cytokinins, auxins and gibberellic acid were increased above 1mg/l there were no germination of seed.Seed germination rate was 90% in 2.5% plain agar medium. Radicle formation was noticed in 2% agar medium without shoot induction. The concentration of agar above 3% were not found to be effective for germination.
Authors and Affiliations
Mrs. Manju Rakesh, Patil N. M.
Keywords
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- EP ID EP395147
- DOI 10.9790/3008-1303025459
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