The Isolation and Structural Determination of Flavonoids from Justicia Gendarussa
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
The current study was intended for isolation of bioactive polyphenolic compounds from methanol extract of Justicia Gendarussa and their subsequent characterization. The flavonoid compounds were isolated and identified by using thin layer chromatography (TLC), were qualified by using reverse phase high performance chromatography (RP-HPLC). Their structures and chemical bonds were analyzed using Ultraviolet-Visible spectrophotometer (UV-VIS spectrophotometer), Fourier Transform-Infra Red spectroscopy (FTIR), Nuclear magnetic resonance NMR (13C and 1H),LC-MS, DSC, SEM and EDX techniques. Two flavonoids were identified as Apigenin and Vitexin. For the first time Apigenin and Vitexin have been isolated successfully from the Justicia Gendarussa. The isolation of the above considered flavonoids would be helpful to practise plant-based pharmaceutical research to treat various difficulties linked with human diseases.
Authors and Affiliations
Raghu. M. G, Pushpa Agrawal2 *
Keywords
Related Articles
- EP ID EP386787
- DOI 10.9790/3008-1106037379
- Views 110
- Downloads 0
Design And Characterisation Of Pulsatile Capsule Device For Flurbiprofen Using Natural Excipients
The objective of the present study was to design and characterize pulsatile capsule device for Flurbiprofen using natural excipients. The Flurbiprofen fast disintegrating tablets (FDT) were formulated using superdisinteg...
New Insights into Aging Process with a Focus on Cell Morphology, Autophagy and Role of Macrophages and NFAT
Aging is a multifaceted process and involves cell senescence and apoptosis. Some key processes which may be involved could be the persistantmorphological changes in the cell structure for e.g cell blebbing and the role o...
Phytochemical Screening And Antibacterial Activity Of Yemeni Henna (Lawsonia Inermis) Against Some Bacterial Pathogens
Phytochemical screening and antibacterial activity of aqueous, methanol extracts of Yemeni henna (Lawsonia inermis) leaves were tested against three bacterial species including (Staphylococcus aureus, Escherichia coli, P...
Efficient Callus Initiation and Plantlet Regeneration of Citrus japonica Margarita
In vitro approaches has become necessary for overcoming the hurdles of cultivation of Citrus, a commercially important fruit. The present study deals with establishment of protocol for micro propagation of Citrus Japonic...
Characterization of Biosurfactant Produced by Pseudomonas putida PS6 Isolated from Contaminated Soil
Biosurfactants are surface active compounds that reduce the interfacial tension between two liquids or between a liquid and solid, these compounds produce extracellularly or as part of the cell membrane in bacteria, yeas...