Phytochemical Screening And Antibacterial Activity Of Yemeni Henna (Lawsonia Inermis) Against Some Bacterial Pathogens
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 2
Abstract
Phytochemical screening and antibacterial activity of aqueous, methanol extracts of Yemeni henna (Lawsonia inermis) leaves were tested against three bacterial species including (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) using agar diffusion and minimum inhibitory concentration (MIC) as a determination method. Preliminary phytochemical screening revealed the presence of Alkaloids, Quinones, Glycosides , Tannins and saponins. The mehanolic extract displayed a potential antibacterial activity against all the bacterial species, than the aqueous extract. The maximum activity was observed in methanolic extract against Staphylococcus aureus at inhibition zone of about (27 ± 1 mm) and minimum activity was observed in aqueous extract against Escherichia coli at inhibition zone of about (8.6 ± 1.2). MIC values for all the existing extracts at a concentration of 2.5mg/ml at Staphylococcus aureus and 10 mg/ml at Pseudomonas aeruginosa.
Authors and Affiliations
Khaled S. Ali, Fuad A. Al-hood, Khaldoon Obad, Mohammed Alshakka3
Keywords
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