Protease Production from Immobilized Bacillus Subtilis and Its Application
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
Biofilm is a complex aggregation of microorganisms growing on a solid substrate. Biofilms are characterized by structural heterogeneity, genetic diversity, complex community interactions, and an extracellular matrix of polymeric substances. Hence adherent cells are frequently embedded within a selfproduced matrix of extracellular polymeric substance (EPS). Biofilms have been found to be involved in a wide variety of microbial infections in the body, by one estimate 80% of all infections. Biofilms have been implicated in urinary tract infections, catheter infections, middle-ear infections, formation of dental plaque, gingivitis, coating contact lenses etc. The attachment cell and free cells from B. subtilis are used for the protease production. The presence of protease was confirmed by protease plate assay in both processes and the enzyme was estimation by biuret method. From the above analysis it is clear that applying this production process through this surface immobilization may suit well in fermentation industry in large scale fluidized bed reactor.
Authors and Affiliations
Dr. K. Jagathy*
Keywords
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- EP ID EP389906
- DOI 10.9790/3008-1204013437
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