Efficient Callus Initiation and Plantlet Regeneration of Citrus japonica Margarita
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 4
Abstract
In vitro approaches has become necessary for overcoming the hurdles of cultivation of Citrus, a commercially important fruit. The present study deals with establishment of protocol for micro propagation of Citrus Japonica Margarita through callus induction and regeneration. Mature seeds of this variety of citrus are used as explant. The Explants were cultured on Murashige and Skoog medium (MS) containing 30 g/L sucrose and 7 g/L agar supplemented with different concentrations and combinations of different phyto-hormones; 6- benzylaminopurine (BA), naphthalene acetic acid (NAA), 2,4-dichloro phenoxy acetic acid (2,4 D). The maximum callus induction (88%) was observed from the mature seeds of Citrus japonica observed on MS medium supplemented with 2,4 D 16 μM. Maximum shoot regeneration response (70%) was observed on MS medium supplemented with BA 13.0 μM. Maximum root regeneration response (80%) was observed on MS medium supplemented with IBA 10 μM and when it is supplemented with NAA 5 μM it is 70%. The regenerated plantlets were successfully acclimatized in pots containing sterile soil mixture to study their response in in vivo conditions.
Authors and Affiliations
Mohammed Raqibul Hasan, Anup Gupta, Md. Nazmul Hasan, Shahariar Mohammed Fahim, Hanif Mohammad Rejwan, Md. Abdullah Shamim, Md. Abu Talha Siddique, Shamsul H. Prodhan1
Keywords
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- EP ID EP385588
- DOI 10.9790/3008-1104037278
- Views 77
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