Effects of Astragalus polysaccharide on immune function in B16 melanoma mice
Journal Title: TMR Integrative Medicine - Year 2021, Vol 5, Issue 12
Abstract
Objective: To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice. Method: Forty male C57BL/6 mice were divided equally into a control group, model group, Astragalus polysaccharide low-dose group, and Astragalus polysaccharide high-dose group, with 10 mice per group. B16 cells were used to develop a mouse model of melanoma. After B16 engraftment, 40 mg/kg and 80 mg/kg Astragalus polysaccharide was administered by gavage every day to the Astragalus polysaccharide low-dose group and Astragalus polysaccharide high-dose group, respectively. Splenic index, thymic index, tumor growth curves, and tumor inhibition rates were measured. Flow cytometry was used to measure proportions of peripheral blood T lymphocyte subsets. Hematoxylin and eosin staining was used to examine histopathological changes in tumors. Immunofluorescence double staining was used to identify myeloid-derived suppressor cells in tumor tissues. Results: In the Astragalus polysaccharide high-dose group, splenic and thymic indices were significantly increased and tumor growth was inhibited in melanoma mice. Flow cytometry demonstrated increased CD4+ and CD4+/CD8+T-cell ratios in the high-dose Astragalus polysaccharide group. HE staining demonstrated significantly decreased numbers of tumor cells among mice with melanoma in the high-dose Astragalus polysaccharide group. Immunofluorescence double staining demonstrated significantly decreased numbers of myeloid-derived suppressor cells in tumor tissues in the high-dose Astragalus polysaccharide group. Conclusion: Astragalus polysaccharide inhibits tumor growth, increases splenic and thymic indices, increases CD4+ and CD4+/CD8+ T-cell ratios, and decreases myeloid-derived suppressor cell numbers in melanoma mice. Our results indicate Astragalus polysaccharide may enhance immune function resulting in inhibition of tumorigenesis and tumor progression.
Authors and Affiliations
Fang-Hua Wu, Kui Chen, De-Kun Zhang, Chao Gong, Jiang-Yue Yu, Shen-Zhou Huang, Chen-Xin Huang, Li-Qun Wang
Keywords
Related Articles
- EP ID EP696227
- DOI 10.12032/TMRIM202105012
- Views 87
- Downloads 0
Acupoint selection rules for acupoint application in lung diseases in the post-epidemic era
Objective: To summarize the rules of acupoint selection of acupoint application to prevent and treat lung diseases under the background the post-epidemic era using data-mining technology. Method: The CNKI, Wanfang databa...
Cinnamomum camphora: a long history for epidemics control
Camphor (C10H16O) is a white crystalline solid exist in enantiomeric form R and S camphor. It is a terpenoid obtained from turpentine oil. Synthetically it is synthesized by catalytic process as alpha pinene. Naturally c...
Analysis of 54 cases of COVID-19
Objective: The clinical features and test results of 2019-nCoV pneumonia (COVID-19) were analyzed retrospectively in order to understand the diagnostic significance of clinical test index, and to summarize the experienc...
Validity of determining innate Mizaj (temperament) based on facial characteristics: a comparative study
In traditional Iranian medicine, accurate determination of Mizaj plays a very important role in the etiology and treatment of diseases. There are a lot of evidences and documents in original Iranian traditional medicine...
Botulinum toxin type A combined with robot-assisted training for upper limb spasticity and motor function after stroke: a case report
This study is to report the clinical experience of of Botulinum toxin type A in combination with robot-assisted training on upper limb spasticity and motor function in stroke patient through a case report. The patient un...