Effects Of Chemically Pretreated Bovine Serum Samples On Sensitivity Of Rose Bengal Test For Serodiagnosis Of Bovine Brucellosis
Journal Title: Journal of Applied Veterinary Sciences - Year 2020, Vol 5, Issue 2
Abstract
The standard serological test such as Rose Bengal Plate Test (RBPT) is routinely used for the diagnosis of brucellosis. This test depends on the agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in sera of infected animals. Faulty negative and positive results are commonly experienced in these traditional agglutination tests. We developed three simple, new, additional and cost-effective steps that can help in these problems; the Superagglutination test for serodiagnosis of brucellosis differs from conventional RBPT by three simple new and coast effective additional steps which are used to overcome this problem. These steps depend on the staining of sera antibodies by adding dye before the test and addition of diluted biotinylated antiglobulin and Avidin in sequence then mixing the antigen with the stained serum. By testing 150 serum samples, the Superagglutination test had higher positive predictive value and specificity than RBPT and standard tube agglutination test (STAT). Also, it had higher negative predictive value and sensitivity than RBPT, STAT, ELISA (Indirect enzyme-linked immunosorbent assay) and CFT (Complement Fixation test).
Authors and Affiliations
Lamees A. El-Tantawy, Lubna, F. Farahat, Eman S. Ramadan, Fatma F. Warda
Effects of Marbofloxacin and Ketoprofen on Some Biochemical and Coagulation Parameters in Calves
Marbofloxacin (MBX) is a 3rd generation fluoroquinolone specifically developed for animal health and is an approved antimicrobial agent for the cure of mastitis and respiratory diseases in cattle and swine. Ketoprofen (K...
Efficacy Of Lyophilized Inactivated Rabies Vaccine
An interesting finding that emerged from the study was the difference in potency between inactivated rabies vaccines prepared in liquid and lyophilized forms. Inactivated rabies vaccine prepared from BHK21 infected cell...
A Comparative Study On Biochemical Parameters For Mature California And Chinchilla Rabbits In Sohag Governorate.
Rabbit as homeothermic species is able to keep its core body temperature within ± 1°C regulatory range. In Upper Egypt where high ambient temperature particularly at summer months, rabbit breeding ceases, by virtue of it...
Potential Health Benefits of Aloe vera in Livestock: A Review
Aloe vera is one of numerous feed supplements that can be used to increase productivity and disease resistance in domesticated animals. To reap these advantages, there are various indications, which include the proper do...
Major Bacterial Challenges Facing Nile Tilapia (Oreochromis niloticus) in Egyptian Fish Hatcheries
Nile tilapia is the most farmed freshwater fish species across vast areas of the African continent. Although Egypt is considered the largest African producer of Nile tilapia, this fish has been faced with frequent episod...