Effects of colchicine on cell cycle arrest and MMP-2 mRNA expression in MCF‑7 breast adenocarcinoma cells (Kolşisin’in MCF-7 insan meme adenokarsinoma hücrelerinde hücre döngüsü tutulumu ve MMP-2 mRNA ifade seviyesi üzerine etkisi)
Journal Title: Turk Hijyen ve Deneysel Biyoloji Dergisi - Year 2018, Vol 75, Issue 3
Abstract
Objective: Colchicine is a tricyclic alkaloid drug and it’s been clinically used for a long time because of its anti-inflammatory effects. While colchicine has been safely used in oral applications, it’s been demonstrated that colchicine is toxic at higher concentrations. So, the mechanism of effects of low dose colchicine has been studied in cancer related researches. Matrix metalloproteinase-2 (MMP-2) is a member of matrix metalloproteinase enzyme family and it’s been demonstrated that MMP-2 expression is increased in cancer and it causes a metastasis of cancer cells through degradation of extracellular matrix. Because of this important effect of MMPs, the studies related to develop MMP inhibitor compounds have great importance in cancer investigations. At present study, it’s aimed to investigate the effects of colchicine on cell cycle arrest and MMP-2 mRNA expression in MCF-7 human breast cancer cells. Methods: In the study, MCF-7 human breast adenocarcinoma cells were purchased from ATCC. Cells were treated with 0.1, 10 ve 100 μg/ml colchicine and cell viability was determined via MTT assay. The effect of colchicine on cell cycle arrest was determined by Muse Cell Analyzer and the percent of cell populations at G0/G1, S and G2/M cycles were identified. The effect of colchicine on MMP-2 mRNA expression has been performed by real-time PCR (qRTPCR) analysis. Results: Colchicine has significantly inhibited cell viability at 10 and 100 μg/ml concentrations. It’s been also demonstrated that colchicine has induced a cell cycle arrest at G2/M phase and downregulated MMP-2 mRNA expression of MCF-7 cells in all treated concentration (p<0.0001). Conclusion: The results of this study illustrated that colchicine may be a candidate anticancer compound for breast cancer studies and further studies are required to identify the underlying mechanism of effects.
Authors and Affiliations
Filiz BAKAR-ATEŞ, Nuri ÖZMEN, Ecem KAYA-SEZGINER, Emin Emre KURT
Keywords
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- EP ID EP484802
- DOI 10.5505/TurkHijyen.2018.22755
- Views 115
- Downloads 0
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