Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2018, Vol 7, Issue 0
Abstract
Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.
Authors and Affiliations
Dion Daniels, Nidia Panti, David Guerra, Stephen Williams
Keywords
Related Articles
- EP ID EP653913
- DOI 10.24297/jbt.v7i1.7567
- Views 60
- Downloads 0
Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455
Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects...
Mutational Analysis of DNASE I Gene in Diabetic Patients
The main aim is to analyze the mutations of DNASE I gene in diabetic patients.A total of 120 diabetes patients and 120 controls were sampled. The total number of male diabetic patients included in the study was 79 (66%)...
Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)
The investigation was carried out to assess the genetic stability in tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Aims:&n...
Development, Optimisation, Validation and Inter-Laboratory Verification of a Reversed Phase HPLC Method for Quantification of Human Recombinant Insulin
HPLC methods for insulin in official monographs require extended runtimes and elevated temperatures. Inter-laboratory reproducibility of HPLC methods obtained from published literature is an on-going challenge, moreso fo...
Effect of Sulphur on Plant Growth & Defense System Against Salinity Stress
The given investigation was undertaken to evaluate the performance of black gram (vigna mungo L.) under different salinity levels (i.e. 50mM, 100mM and 150mM NaCl) and to find out the remedial effect of two doses of S (i...