Effects of endothelin-1 on prevention of microvascular endothelium injuries in hemorrhagic shock in rats.
Journal Title: Pharmacological Reports - Year 2007, Vol 59, Issue 1
Abstract
The aim of this study was to examine the effects of posthemorrhagic hypovolemia and hypotension upon the microvascular endothelial cells and on activity of antioxidant enzymes in blood, and to investigate the influence of intravenously injected endothelin-1 in rats. The experiment was conducted on 48 rats anesthetized with ethylurethane, subjected to controlled hypotension (under 35-40 mmHg) for 60 min. Endothelin-1 was administered intravenously once at a dose of 50 pmol/kg in the 5th min of hemorrhagic shock. The control group had blood volume restored after 5 min of hypovolemia with hypotension. The arterial blood pressure, systolic and diastolic, and heart rate were monitored. After 60 min, morphological changes in the capillary endothelium of the small intestine were assessed, using electron microscope, and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) in blood were measured. Animals with hypovolemia and hypotension, had edematous endothelial cells with injured cell-membrane and mitochondria, alongside of the enhancement in SOD activity (p < 0.05) and drop in the activity of CAT and GSH-PX. No signs of vascular endothelium injuries and no reduced enzymatic activities, except for GSH-PX, were observed after restoring the normal blood pressure by means of endothelin-1 in animals with hypovolemia. Hemorrhagic shock caused injuries in intestinal microcascular endothelium. Intravenously administered endothelin-1 quickly restored normal blood pressure, maintained it over along time, and prevented the consequences of ischemia in microcirculation, thereby prolonging the survival for animals in hemorrhagic shock.
Authors and Affiliations
Ilona Korzonek-Szlacheta, Bolesław Gwóźdź
Keywords
Related Articles
- EP ID EP144414
- DOI -
- Views 82
- Downloads 0
CYP2D6 phenotyping with dextromethorphan.
Genetically determined individual differences in the ability to oxidize certain drugs have raised recently a considerable interest because of clinical importance of this problem. Determination of CYP2D6 oxidation phenoty...
Preoperative tramadol combined with postoperative small-dose tramadol infusion after total abdominal hysterectomy: a double-blind, randomized, controlled trial.
This double blind, randomized, controlled trial investigated whether a single preoperative intravenous (iv) dose of tramadol (100 mg) given 30 min before abdominal hysterectomy resulted in improved analgesic efficacy, re...
Hemostatic effects of fenofibrate in patients with mixed dyslipidemia and impaired fasting glucose.
Our study aimed to compare the effect of fenofibrate on hemostasis between patients with isolated impaired fasting glucose (IFG) and isolated mixed dyslipidemia and to examine the action of this agent on glucose and lipi...
Enhancement of fibrinolysis by gel-filtered platelets and its quenching by cytochalasin B and GPIIb/IIIa antagonists.
The effects of gel-filtered platelets on euglobulin clot lysis time (ECLT) were analyzed to elucidate the possible role of platelets in thrombolysis. Gel-filtered platelet-supplemented ECLT (plt-ECLT) was significantly s...
Down-regulation of thymic stromal lymphopoietin by curcumin.
Background: Thymic stromal lymphopoietin (TSLP) is a cytokine implicated in the pathogenesis of allergic diseases such as asthma, atopic dermatitis and allergic rhinitis. Curcumin has various effects such as antidepressa...