Down-regulation of thymic stromal lymphopoietin by curcumin.
Journal Title: Pharmacological Reports - Year 2013, Vol 65, Issue 2
Abstract
Background: Thymic stromal lymphopoietin (TSLP) is a cytokine implicated in the pathogenesis of allergic diseases such as asthma, atopic dermatitis and allergic rhinitis. Curcumin has various effects such as antidepressant, antioxidant, antihyperglycemic, antitumor and anti-inflammatory. However, the effect of curcumin on the production of TSLP has not been clarified. Thus, we investigated how curcumin inhibits the expression and production of TSLP in the human mast cell line, HMC-1 cells. Methods: We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and caspase-1 assay to investigate the effects of curcumin. Results: The results show that curcumin inhibited the production and mRNA expression of TSLP in HMC-1 cells: the maximal inhibition rate of TSLP production by curcumin (50 μM) was 59.16 ± 4.20%. In addition, curcumin suppressed the nuclear factor-κB luciferase activity induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, caspase-1 activity was increased, whereas caspase-1 activity was decreased by pretreatment with curcumin. Conclusion: These results suggest that curcumin can be used to treat inflammatory and atopic diseases through the suppression of TSLP.
Authors and Affiliations
Phil-Dong Moon, Hyun-Ja Jeong, Hyung-Min Kim
Keywords
Related Articles
- EP ID EP104114
- DOI -
- Views 113
- Downloads 0
Ephedrine-caffeine mixture in wet-cold stress.
Our investigations were aimed at studying the possibility of enhancement of homeostatic processes protecting against excessive body cooling by using thermogenic drugs. We studied the influence of ephedrine (1 mg/kg) and...
Effect of diazepam and chlorpromazine on proliferative activity and vascular endothelial growth factor (VEGF) secretion from cultured endothelial HECa10 cells in vitro.
Diazepam and chlorpromazine - psychotropic agents widely used for the amelioration of anxiety, depression, and psychosis, have been found to affect cell proliferation. We have checked whether these drugs influence prolif...
Omeprazole does not change the oral bioavailability or pharmacokinetics of vinpocetine in rats.
Previous studies proved that food strongly enhanced the bioavailability of vinpocetine. Food may change the pharmacokinetics of a drug by affecting various factors, including gastrointestinal pH. However, the influence o...
Effects of the antioxidant baicalein on the pharmacokinetics of nimodipine in rats: a possible role of P-glycoprotein and CYP3A4 inhibition by baicalein.
The reduced bioavailability of nimodipine after oral administration might not only be due to the metabolizing enzyme cytochrome P450 3A4(CYP3A4) but also to the P-glycoprotein efflux transporter in the small intestine. T...
In vivo and ex vivo responses of CLL cells to purine analogs combined with alkylating agent.
Background: The heterogeneity of chronic lymphocytic leukemia (CLL) is thought to be due to differences in the expression of factors that regulate apoptosis and cell cycle, giving rise to diverse apoptotic disturbances a...