EFFECTS OF ENHANCED DULBECCO′S MODIFIED EAGLE MEDIUM ON BLASTOCYSTIS HOMINIS CULTURE
Journal Title: Acta Parasitologica et Medica Entomologica Sinica - Year 2024, Vol 31, Issue 2
Abstract
[Objective] To investigate the effect of enhanced Dulbecco′s modified Eagle′s medium (DMEM) on the cultivation of Blastocystis hominis. [Methods] A total of 329 clinical samples were selected to compare the growth of B. hominis using enhanced DMEM and the iodine solution direct smear method. The morphology, minimum detection limit and growth curve of B. hominis were compared between the conventional DMEM culture medium and the enhanced DMEM culture method. [Results] In 329 clinical specimens, the positive rate for B. hominis was 5.17% (17/329) in the enhanced DMEM culture and 1.52% (5/329) in the iodine solution stained smears; the difference was statistically significant (P < 0.01). [Conclusion] The enhanced DMEM culture method can be used for the proliferation and culture of B. hominis in vitro.
Authors and Affiliations
Ming-chao ZHU, Ya ZHU, Hui-zhen YUAN, Fei-bo GUO, Jun WANG, Ling SHU
Keywords
Related Articles
- EP ID EP753981
- DOI 10-3969/j.issn.1005-0507-2024-02-002
- Views 26
- Downloads 0
ANALYSIS OF K26 GENE AND ITS-1 SEQUENCE POLYMORPHISMS IN THE REEMERGING CASE OF VISCERAL LEISHMANIASIS IN BEIJING
[Objective] To understand the molecular genetic background of the reported case of reemerging visceral leishmaniasis in Beijing. [Methods] The PCR was used to amplify the hydrophilic acylated surface protein B (K26) gene...
SURVEILLANCE ON MAJOR HUMAN PARASITIC DISEASES AND RESIDENT AWARENESS IN TONGZHOU DISTRICT, BEIJING FROM 2011 TO 2022
[Objective] To analyze the surveillance and investigation results of parasitic diseases in the Tongzhou district of Beijing from 2011 to 2022, and to provide scientific basis for formulating prevention and control measur...
MOLECULAR EPIDEMIOLOGICAL INVESTIGATION ON CO-INFECTION OF INTESTINAL PROTOZOA IN GASTROINTESTINAL CANCER PATIENTS
[Objective] The relationship between parasitic infections and cancer has become a research hotpot. Although reports of single intestinal protozoan infection in gastrointestinal cancer patients, co-infections are rare. To...
RESEARCH PROGRESS ON INNATE IMMUNE REGULATORY MECHANISMS IN INSECTS
Insects frequently encounter microbial infections in their natural environment. Infections such as viruses carried by vector arthropods can cause serious human illness or animal epidemics. However, insects rely primarily...
FIELD CONTROL EFFICACY OF S-METHOPRENE ON GERMAN COCKROACHES, BLATTELLA GERMANICA IN SHANGHAI CITY
[Objective] A field trial was conducted to explore the efficacy of S-methoprene for controlling German cockroaches. [Methods] Two farmer's markets in Shanghai City were randomly selected as locations for conducting our r...