Effects of mixed cultivation mode and nutrient condition optimization on biomass accumulation and lipid content of Tetradesmus obliquus
Journal Title: Energy Environmental Protection - Year 2024, Vol 38, Issue 6
Abstract
Microalgae are considered to be the most promising feedstock for biodiesel production due to their advantages in high photosynthetic efficiency, growth rate, and lipid accumulation rate, which can alleviate the environmental pollution caused by fossil fuels. To increase the biomass, lipid content, and high-value product yield of microalgae, it is necessary to optimize their cultivation modes and nutritional conditions. This study investigated the optimal cultivation mode for the growth of Tetradesmus obliquus under three conditions: autotrophic, mixotrophic, and heterotrophic. Additionally, response surface methodology was employed to optimize the nutrient conditions for cultivation, with the goal of improving the biomass and lipid content of microalgae. The results showed that mixotrophic cultivation was the most suitable mode for the growth and development of Tetradesmus obliquus. Under mixotrophic cultivation conditions for 24 hours, the biomass and lipid content of microalgae were 3.82 times and 2.49 times higher, respectively, compared to autotrophic cultivation conditions. Moreover, the highest protein production during the logarithmic growth phase was the highest in the 8-hour mixotrophic and 16-hour autotrophic combination, reaching 554.87 mg·Lâ1. By optimizing the nutritional substances using response surface methodology, the optimal parameters under mixotrophic conditions were as follows: Carbon-to-nitrogen ratio of 10, trace element addition of 0.1 mL, and vitamin addition of 1.0 mL. The measured biomass and predicted values were 2.45 g·Lâ1 and 2.43 g·Lâ1, respectively. The optimization of mixotrophic cultivation mode and nutritional conditions could enhance microalgae biomass and utilization efficiency.
Authors and Affiliations
Shuyuan LI,Dan ZHAO,Canmei WANG,Ting YUAN,Xiangmeng MA,Qun WEI,
Keywords
Related Articles
- EP ID EP761355
- DOI -
- Views 12
- Downloads 0
Research on the performance of Cu(X)/SSZ-13 zeolite catalysts with different Cu contents for simultaneous elimination of soot and NO_x
Diesel engines have been widely applied in heavy industries such as transportation, agriculture, and construction due to their advantages of strong power and durability. However, the pollutants emitted by diesel engines,...
Analysis of material flow and energy flow in collaborative pyrolysis of Chinese medicine residue
Pyrolysis is a mainstream technology for biomass energy utilization, but theoretical simulation, verification feedback on the coordinated pyrolysis process, material conversion rules, and energy efficiency characteristic...
Denitrification research based on green electron supply and cell metabolism regulation
As a key link in the nitrogen cycle, microbial denitrification plays an important role in removing nitrate pollution from the water environment, realizing the return of nitrogen to the atmosphere, and maintaining nitroge...
Treatment of large-scale mine drainage with high suspended solid and fluoride by the process of two-stage preprecipitation and mechanical clarifier: A case study of Zhengtong coal mine
Treatment and resource utilization of mine drainage is an effective way to protect the ecological environment and address the issue of water shortage in mining areas. According to the characteristics of mine drainage of...
Research progress on emission inventory of anthropogenic air pollutants in China
The air pollutant emission inventory can not only provide the basic information of air pollutant emission sources to facilitate the decision-making of air pollution prevention and control, but also provide essential data...