Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation
Journal Title: Journal of Health Science and Medical Research (JHSMR) - Year 2019, Vol 37, Issue 2
Abstract
Objective: To compare human sperm motility, vitality and morphology after cryopreservation among sucrose, and different trehalose concentrations. Material and Methods: A total number of 124 normozoospermic semen samples were collected. Each semen sample was divided into 4 portions, and cryopreserved in a human sperm-preserving medium along with cryoprotectants, including; 50 milimolar (mM) trehalose, 100 mM trehalose, 200 mM trehalose and 50 mM sucrose, respectively. All semen samples were frozen by using a vapor phase method. Post-thawed sperm motility, vitality and morphology were assessed. R program was used for data analysis. A p-value of <0.05 was considered statistically significant. Results: Post-thawed semen evaluation indicated that 50 mM trehalose was better than 50 mM sucrose in all sperm parameters, which included progressive motility (p-value=0.037, total motility (p-value<0.001), vitality (p-value<0.001) and morphology (p-value<0.001). The sperm parameters were not significantly different among 100 mM trehalose, 200 mM trehalose and 50 mM sucrose. Conclusion: The use of 50 mM trehalose, as non-permeating cryoprotectant, showed superior post-thaw sperm parameters over sucrose, and other trehalose concentrations.
Authors and Affiliations
Manaphat Suksai, Kriengsak Dhanaworavibul
Keywords
Related Articles
- EP ID EP678155
- DOI http://dx.doi.org/10.31584/jhsmr.201945
- Views 193
- Downloads 0
Improvement of the Osmotic Fragility (OF) Test in α Thalassemia 1 Screening for HIV-1 Infected Patients by The New Cutoff Values of MCV and MCH
Objective: To evaluate the efficiencies of the standard α thalassemia screening regimen in human immunodeficiency virus (HIV) infected patients and improve the efficiencies of this screening regimen using new cutoff valu...
A Study of Entrance Surface Air Kerma for Patients Undergoing Chest and Abdomen from Digital Radiography at Chulabhorn Hospital
Objective: The main purpose of this study was to investigate the typical dose for standard-sized patients in chest (posteroanterior; PA) and abdomen (anteroposterior; AP) digital radiography. Material and Methods: The a...
Validation of Functional Assessment of Cancer Therapy with Nasopharyngeal Cancer Subscale (FACT-NP) for Quality of Life in Thai Patients
Objective: To develop and validate the Thai version of the functional assessment of cancer therapy with nasopharyngeal cancer subscale (FACT-NP) with the abbreviated version of the World Health Organization Quality of Li...
Serum Sodium Levels Predict the Recurrence of Febrile Seizure within 24 Hours
Objective: Febrile seizure is a common disorder in children that occurs in 2.5% of children 6-60 months of age. The study was conducted to ascertain the role of serum sodium as a predictor of seizure recurrence within th...
Perioperative Risk Factors for Intraoperative Hypothermia in Adult Patients Undergoing Elective Surgery at a National Referral Hospital in Bhutan: A Prospective Observational Study
Objective: Intraoperative hypothermia is commonly encountered in anesthetic practice. It is related to several risk factors and can lead to various adverse events. It is important to detect it early and prevent the compl...