Effects of Various Levels of Luteinizing Hormone and Caprine Follicular Fluid on In Vitro Embryo Production of Shami Goat
Journal Title: ISPEC Journal of Agricultural Sciences - Year 2021, Vol 5, Issue 3
Abstract
In the current study, the hypothesis of the effects of luteinizing hormone (LH) and follicular fluid (FF) derived from follicles of varying size on in vitro embryo production of the Shami goat breed were tested. The caprine follicular fluid (cFF) was obtained from healthy female’s ovaries by aspiration method and classified into two main classes (follicles with a diameter of ≤ 2mm and ≥3mm). The resulting cFF was added to the culture medium TCM-199 through six Treatments (A, B and C with a source of follicle size of ≤ 2mm; D, E and F with a source of size of ≥3mm). LH was added only to four of the previous Treatments with the levels of 50 µg ml-1 (B and E) and100 µg ml-1 (C and F). Results of the study showed that the oocytes incubated in Treatment F achieved a clear superiority (p=0.001) in the rates of maturation (87.0%), fertilization (80.0%) and cleavage (82.3%). The oocytes incubated in the same Treatment (F) continued to outperform (p= 0.006) by achieving the best rates across cleavage stages at 2-16 cell (16%; the lower value of arrest) and blastocyst (42%). Significant differences (P=0.03) were observed among the rates of Type 1embryos (the highest rate: 45.3%; Treatment F) and Type 3 embryos (the highest rate: 45.1%; Treatment A). No significant differences were observed in the rates of morula and Type 2 embryos. It is advised to add 15% of the cFF derived from follicles with a diameter of ≥3mm and 100 µg of LH ml-1 in the maturation media to obtain higher rates of maturation and cleavage of goat oocytes.
Authors and Affiliations
Omar Mardenli, Hadi Awad Hassooni, Mahdi Saleh Mohammad Al-Kerwi
Keywords
Related Articles
- EP ID EP725585
- DOI https://doi.org/10.46291/ISPECJASvol5iss3pp575-584
- Views 24
- Downloads 0
The Effect of Potassium and Calcium Fertilization on the Quality Parameters and K and Ca Content of Lilium (Lilium casablanca) Plant
This study was carried out to determine the effects of Lilium (Lilium casablanca) ornamental plant on the quality parameters and potassium and calcium contents of potassium and calcium fertilizers. Randomized blocks desi...
Effects of Normal and Ultra-Narrow Twin Row Planting Methods on Yield and Some Fiber Properties in Cotton (Gossypium hirsutum L.)
Cotton is the most important plant for natural fiber production. Cotton cultivated in Turkey with mechanized methods, are plant in 70 x 15 cm2 sowing. This study was conducted in Kahramanmaraş in 2019 in order to determi...
Evaluation of the In Vitro Fungicidal Activity of Summer Savory and Lavender Essential Oils Against Fusarium solani
Fusarium spp is one of the major phytopathogenic microfungus strains causing severe losses in many economically cultivated crops. The soil-borne pathogen Fusarium solani has historically been considered a serious agent a...
Impact of Paper Waste and Earthworm on Nutrient and Heavy Metal Content of Rice Straw Compost in the Absence of Manure
Plant residues after harvest are serious problem. In the recycling process of these residues, composting and vermicomposting is an economic, ecologic, and biologic alternative. Recycling of rice straw waste is also diffi...
Determination with Correlation and Path Analysis of Relationships between Dry Matter Yield with some Yield Components and Quality Characteristics in Buckwheat (Fagopyrum esculentum Moench)
This study was carried out Agricultural Application and Research Center of the Faculty of Agriculture of Bursa Uludağ University in 2018 and 2019. The experimental design was the Randomized Complete Blocks with three rep...