Efficacy of Chloroquine against Escherichia Coli and Proteus vulgaris: An in vitro Study
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2014, Vol 2, Issue 6
Abstract
Chloroquine, a 4-aminoquinoline is an effective antimalarial drug and is also used as a systemic amoebicide in the treatment of hepatic amoebiasis (extra intestinal site). The objective of this study is to establish the efficacy of Chloroquine against common pathogenic bacteria such as Escherichia coli, and proteus. A stock solution containing 64 mg Chloroquine phosphate/mL was prepared in distilled water, serial dilution was prepared to obtain 5 mL solution, each having a different concentration of the drug. Whatman No.1 filter paper was used to prepare discs of 6mm diameter. The discs were sterilized and each dilution of the drug was added on the disc at a volume of 10µL per disc. Final concentration of Chloroquine phosphate per disc was 64, 53, 42, 30 and 21µg. Discs were dried and stored at 4oC. Disc of amoxicillin-clavulanic acid combination at strength of 30µg was used as a control. Total 100 isolates of bacteria isolates from clinical samples was used in the study. Antimicrobial susceptibility test of these isolates was performed by using Kirby-Bauer’s method. It was observed that E. coli and Proteus were susceptible to chloroquine at higher strengths. Interpretation and chloroquine certainly shows certain degree of antimicrobial efficacy. In-vitro studies can be a stepping stone for further investigations in-vivo. Keywords: Chloroquine, Antimicrobial Susceptibility Test, Efficacy, E. coli, Proteus
Authors and Affiliations
Jagadeesh K, Saivisveswar. K. N, Shreenivas P Revankar
Keywords
Related Articles
- EP ID EP375727
- DOI -
- Views 38
- Downloads 0
Co-relation between Morphology and Special Stains with Flow Cytometry in the diagnosis of Acute Leukemia
Abstract: Acute Leukemia accounts for approx 0.15-0.60% of total medical admissions in hospitals in India. It is still diagnosed mainly on the basis of morphology and cytochemistry in many centres, because of its ease an...
Oral Hygiene of Children Talibes of the Prefecture of Banamba in Mali: 205 Observations
Abstract: According to WHO, oral health is an essential and integral component of health in general. Alterations in oral health have important consequences: chewing, slurred speech, sleep and concentration, aesthetics an...
Study on Incidence, Types and Treatment Options of Carcinoma Penis
Abstract: Carcinoma penis is a common malignancy. Accounting for upto 10% of all cancers in uncircumcised male population in Asian countries. Carcinoma penis is almost unheard of races like the jews, who practice neonata...
Refractory hypokalemia as initial manifestation of Cushing’s syndrome complicating pregnancy
A pregnancy complicated by Cushing’s syndrome is a high-risk obstetric condition. The overlap in clinical features with pregnancy, as well as its rare occurrence, makes the diagnosis of Cushing’s syndrome difficult and o...
An Unusual Presentation of Gallbladder Carcinoma-Gallbladder Perforation and Anterior Abdominal Wall Abscess
Gallbladder perforation (GBP) is a rare but life threatening complication of acute cholecystitis with high morbidity and mortality rates because of delay in diagnosis. Most cases can only be diagnosed during surgery. Ni...