Efficacy of Chloroquine against Escherichia Coli and Proteus vulgaris: An in vitro Study

Apply

Efficacy of Chloroquine against Escherichia Coli and Proteus vulgaris: An in vitro Study

Journal Title: Scholars Journal of Applied Medical Sciences - Year 2014, Vol 2, Issue 6

Abstract

Chloroquine, a 4-aminoquinoline is an effective antimalarial drug and is also used as a systemic amoebicide in the treatment of hepatic amoebiasis (extra intestinal site). The objective of this study is to establish the efficacy of Chloroquine against common pathogenic bacteria such as Escherichia coli, and proteus. A stock solution containing 64 mg Chloroquine phosphate/mL was prepared in distilled water, serial dilution was prepared to obtain 5 mL solution, each having a different concentration of the drug. Whatman No.1 filter paper was used to prepare discs of 6mm diameter. The discs were sterilized and each dilution of the drug was added on the disc at a volume of 10µL per disc. Final concentration of Chloroquine phosphate per disc was 64, 53, 42, 30 and 21µg. Discs were dried and stored at 4oC. Disc of amoxicillin-clavulanic acid combination at strength of 30µg was used as a control. Total 100 isolates of bacteria isolates from clinical samples was used in the study. Antimicrobial susceptibility test of these isolates was performed by using Kirby-Bauer’s method. It was observed that E. coli and Proteus were susceptible to chloroquine at higher strengths. Interpretation and chloroquine certainly shows certain degree of antimicrobial efficacy. In-vitro studies can be a stepping stone for further investigations in-vivo. Keywords: Chloroquine, Antimicrobial Susceptibility Test, Efficacy, E. coli, Proteus

Authors and Affiliations

Jagadeesh K, Saivisveswar. K. N, Shreenivas P Revankar

Keywords

Comparative evaluation of one stage versus two stage surgical procedure for surgical extraction of bilaterally impacted mandibular third molars

A tooth which is completely or partially unerupted and is positioned against another tooth, bone or soft tissue so that its further eruption is unlikely is called an impacted tooth. The present study of comparative evalu...

A Comparative Study of Drainage of Breast Abscess by Conventional Incision and Drainage versus Percutaneous Placement of Suction Drain

Abstract: Breast abscess is common in puerperal period secondary to mastitis with incidence of 4.8%-11%. The conventional method of incision and drainage of the breast abscess has undergone a gradual change from invasive...

EP ID EP375727
DOI -
Views 50
Downloads 0