Electrophoretic Analysis of Erythrocyte Proteins in Neonatal Jaundice
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2019, Vol 5, Issue 1
Abstract
Neonatal jaundice is an alarming situation manifested by hyperbilirubinemia in early infancy. In the present study we analysed the proteins using electrophoretogram in erythrocyte lysate of neonatal jaundice in comparison with normal control. From the elctrophoretogram we observed there was a decrease in band intensity corresponding to catalase and pyruvate kinase monomers. Besides, there were other proteins such as peroxiredoxin and haemoglobin showed marginal decrease at their respective bands in neonatal jaundice hemolysates in comparison to control and the band in the region 260 KDa showed higher band intensity in neonatal jaundice sample compared to control in the electrophoretogram which could be spectrin. The catalase activity by spectrophotometry also observed there was lowered catalase activity in both hemolysate and plasma of neonatal jaundice compared to control.
Authors and Affiliations
Dr. Mathew John, Ms. Aghimol Varghese, Ms. Evelyn Maria, Ms. Sinju. R
Keywords
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- EP ID EP443092
- DOI 10.9790/264X-0501011014.
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