Encapsulation of Streptococcus Salivarius in Double Emulsion Droplets as a Method for Increasing the Efficacy of Oral Topical Medications
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 19, Issue 2
Abstract
Streptococcus alivarius, a flora present in the human oral cavity, plays an important role in wound healing. This study aimed to optimize double emulsion droplets (DEDs) to encapsulate Streptococcus Salivarius with increased viscosity and adhesiveness as an oral topical medication. Streptococcus Salivarius grew nearly as well in saliva (68%) as in broth and grew significantly better than the control (p < 0.05) over 72 hour. The diameter of DED according to 10 weight% PVA and 15 weight % n-hexadecane span 80 was the largest. The percentage of the ointment absorbed into the oral epithelium was 88.44% of DEDs for optimal 80% formulation compared to 64.63% the control containing 0% DEDs. Double emulsion droplets that encapsulated S. Salivarius increased the efficacy of the topical medication in the oral cavity and improved wound healing in the oral cavity.Good oral health is critical for general health and well-being. Several innovative drug delivery systems have been developed for local treatment of various oral diseases [1]. However, these delivery systems exhibit limited efficacy and retention at the target site [2]. Bio-adhesive formulations may be a potential mechanism to improve drug delivery to the oral cavity. Probiotics enhance mucosal barrier function and promote intestinal epithelial survival and growth [3-5]. Probiotic bacteria in the skin and in the oral cavity have received increasing attention [6,7]. Recent studies have evaluated the antimicrobial activity of Lactobacillus, and the ability of Lactobacillus to promote host-microbe homeostasis [8- 10]. Streptococcus Salivarius is one of the first colonizers of the human oral cavity. It plays an important role in wound healing and anti-inflammatory processes in human intestinal epithelial cells [11]. Several strains of S. Salivarius have been shown to stimulate re-epithelialization in the gingiva. Further analysis showed that S. Salivarius significantly promoted re-epithelialization through a secreted proteinaceous compound. S. Salivarius also was attempted to use as probiotics to prevent fungal infection. Water-in-oil-in-water double emulsions are dynamic systems with potential applications in many fields. These emulsions are characterized by water droplets dispersed within oil droplets, which are then dispersed within a secondary water phase. These emulsions have high encapsulation potential, and represent a simple, low-cost method to increase viscosity. Double emulsions also can effectively mask undesirable tastes. Previous studies have demonstrated encapsulation of iron or milk fat within double emulsions [7-11]. Encapsulation of S. Salivarius using double emulsions may be a potential oral therapeutic strategy. These double emulsions may allow for the targeting of specific wounds using hydrophilic properties and may improve the taste and texture of current medications [12]. Furthermore, double emulsions may improve drug absorption and adhesion. This study aimed to optimize double emulsion droplets (DED) to encapsulate S. Salivarius. We confirmed that the optimized DED had increased viscosity and adhesiveness in the oral cavity.
Authors and Affiliations
Seung Ah Choi, Soonmin Lee
Keywords
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- EP ID EP620686
- DOI 10.26717/BJSTR.2019.19.003271
- Views 159
- Downloads 0
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