Endothelial nitric oxide synthase 4a/b polymorphism and its interaction with g894t variants in type 2 diabetes mellitus: predicting the risk of diabetic nephropathy
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH - Year 2016, Vol 8, Issue 12
Abstract
Background: Nitric oxide (NO), a vasodilator molecule, is produced from L-arginine by endothelial nitric oxide synthase (eNOS) and it regulates endothelial function. The presence of eNOS variants might further complicate endothelial dysfunction and nephropathy through reduced production of NO. This study aimed to find out the possible association and synergistic effect of eNOS 4a/b and G894T polymorphisms on the risk of diabetic nephropathy. Patients and Methods: This study conducted on 45 patients with diabetes mellitus type 2 and 15 healthy subjects serving as a control group. All candidates were genotyped for the eNOS polymorphism using PCR-RFLP technique. Results: there are significant differences in genotype and allele frequencies between patients and control for eNOS G894T and (4a/b) Polymorphisms with p value ˂0.05, and It also showed significant differences between patients with DN and patients without DN for the GT genotype, a and T alleles with p value ˂0.05. The subjects with GT or aa genotypes were associated with risk to develop micro and macroalbuminuria when compared with controls but the presence of both them had no significant effect. Conclusion: Analyzing both eNOS 4a and 894T alleles revealed that in the presence of either eNOS 4a or 894T allele, the risk of developing DN increased significantly. However, the presence of both alleles together was not associated with increased risk of microalbuminuria and macroalbuminuria.
Authors and Affiliations
Azza Abo Senna, Jehan H Sabry, Mohamed A Al Hindi, Hesham A Eissa -, bdelmonem Ahmed Basma M Salem
Keywords
Related Articles
- EP ID EP405027
- DOI -
- Views 111
- Downloads 0
Plasma Cell Mucositis Of Lower Lip– A Rare Case Report With Literature Review
Plasma cell mucositis is a chronic, multifocal, non-neoplastic plasma cell proliferative disease of upper aerodigestive tract having unknown etiology, very rare condition with less than 50 cases reported in literature ti...
Age estimation using chronological changes in the epiphyseal ends of the clavicle
The determination of an individual’s age is an important aspect of forensic medicine. The clavicle has the longest period of growth; therefore, it has been investigated by various researchers in order to establish a reli...
Mucoid variant of pseudomonas aeruginosa but not in cystic fibrosis lung
Mucoid variant of Pseudomonas is most commonly isolated from Cystic Fibrosis patients. We have isolated a hugely mucoid variant of it from sputum of two non-cystic fibrosis patients, one aged 75 years and another aged 35...
Histopathological signs and their relationships with survival in chronic allograft nephropathy
Aim: To investigate possible factors contributing to chronic allograft dysfunction (CAD), efficacy of histopathological evaluation in recipients with chronic allograft nephropathy (CAN), and determinative role of histopa...
Pregnancy Complicating Patent Ductus Arteriosus With Eisenmenger Syndrome
Congenital heart disease (CHD) is associated with significant morbidity and mortality in pregnancy (Whittemore et al., 1982 and Shime et al., 1987). Congenital heart disease in pregnancy is associated with reduced cardi...