Enhancement Of Pneumo-4 Vaccine Efficacy By Using Of Carbomer As An Adjuvant
Journal Title: Journal of Applied Veterinary Sciences - Year 2019, Vol 4, Issue 2
Abstract
The present study aimed to prepare a combined inactivated pneumo-4 vaccine containing bovine viral diarrhea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV) adjuvanted with carbomer and compare the animal protection scope with its product mate Pneumo-4 adjuvanted alhydragel . Carbomer adjuvant stimulated higher levels and longer-lasting antibody to the pneumo-4 vaccine than vaccines of equivalent antigenic content containing aluminum hydroxide gel adjuvant. Quality control results proved that the pneumo-4 vaccine adjuvanted either by aluminum hydroxide gel adjuvant or carbomer (different concentrations 0.2,0.5 and0.8 %) was pure and completely safe to be used in calves without any abnormalities. Potency test was performed on five groups of five calves for each group, where the first group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.2(formula 2), second group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.5 (formula 3), third group was vaccinated with pneumo-4 vaccine adjuvant with carbomer 0.8(formula 4),, The fourth group vaccinated with pneumo-4 vaccine with aluminum hydroxide gel(formula 1), and the fifth last group was left as non vaccinated control group. compared humoral immune response using serum neutralization and ELISA expressed by neutralization index of different vaccine formulation was revealed that The prepared pneumo-4 vaccine adjuvant with carbomer 0.5 proved to be highly potent formulas along 6 months after second booster dose applying. Also, pneumo-4 vaccine adjuvant with carbomer was showed cellular immunity reaction higher than pneumo-4 vaccine with aluminum hydroxide gel using Estimation of nitric oxide activity test.
Authors and Affiliations
Maha Raafat Abed EL-Fadil*, Rasha, I. EL-Hawary,Walaa. S. Shabana , Ekbal, M. Farok and Naglaa,I. Ali
Keywords
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- EP ID EP677470
- DOI 10.21608/JAVS.2019.62653
- Views 184
- Downloads 0
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