Enterococcus faecalis biofilm formation on gutta-percha obturating material coated with or without sealer and conditioned using saliva or serum - an in vitro study.
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 10
Abstract
Introduction: Presence of biomaterials in close proximity to the host immune system increases the susceptibility to biofilm formation. It could provide surface for bacterial adherence and formation of biofilm - leading to biomaterial-centered infections. Gutta percha is used as a root canal obturating biomaterial. The aim of this study is to assess and compare an Enterococcus faecalis (E Faecalis) biofilm formation on saliva/serum conditioned specimens of Gutta percha (GP) points coated with calcium hydroxide or zinc oxide eugenol sealer. Methodology: Gutta percha point samples were divided into six groups based on the combinations of root canal sealers and conditioning liquids. Polyethylene tubes were used to prepare samples coated with sealer. Specimens, conditioned in saliva/serum for fifteen and thirty days, were incubated with Enterococcus faecalis for a period of 14 days in nutrient-rich media. Viable cell assay & scanning electron microscope examination was used to assess biofilm formation. Data obtained was statistically analyzed using ANOVA and Duncan’s multiple range test. p-value <0.05 was considered as statistically significant. Results: Statistically significant difference between the groups was observed (p=0.002). Saliva conditioned specimens of Gutta Percha points without sealer showed maximum mean Colony Forming Unit (CFU) count whereas Sealapex coated Gutta Percha points conditioned using serum for 15 days showed least biofilm formation. Conclusion: All GP specimens exhibited biofilm formation. Conditioning medium, duration, and sealer affect biofilm formation.
Authors and Affiliations
Dr Mantri Shivkumar P.
Keywords
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