Enumeration, identification, and antibiogram of some pathogenic bacteria recovered from diseased farmed Clarias gariepinus (Burchell, 1822)
Journal Title: Journal of Applied Veterinary Sciences - Year 2024, Vol 9, Issue 4
Abstract
Prevalent etiological agents and treatment options are essential for diagnosing and managing fish diseases. Therefore, in this study, enumeration and identification of the total bacterial count in water, skin, and gastrointestinal tract of 75 moribund cultured Clarias gariepinus from different culture facilities were investigated using standard microbiological procedures. Following that, the antibiotic susceptibility of the isolated bacteria to eight antibiotics was tested using the Kirby-Bauer disc diffusion method. Various clinical signs and pathological lesions were observed on the sampled moribund fish. Total bacterial count varied significantly (P < 0.05) regardless of the types of culture facilities. Bacteria species from eight different genera were present, comprising three Gram-positive bacteria; Bacillus subtilis, Streptococcus agalactiae, and Staphylococcus aureus; and five Gram-negative bacteria; Aeromonas hydrophila, Citrobacter freundii, Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica were identified in which Gram-negative bacteria (> 70 %) were the most prevalent type of bacteria isolated. There was an association between the bacteria species isolated from the water and the different parts of the fish sampled regardless of the culture facilities. The antibiogram of the bacteria recovered revealed different sensitivity and resistance profiles. More than fifty percent of the Gram-negative bacteria were sensitive to ciprofloxacin, while the Gram-positive bacteria were susceptible to ciprofloxacin and gentamycin (> 40 %). Amoxicillin, ampicillin oxytetracycline, and tetracycline resistance were high in the bacteria isolates. This study confirmed the presence of antibiotic-resistant pathogenic bacteria isolated with a multiple antimicrobial resistance (MAR) index of 0.63 - 0.75, indicating indiscriminate and irrational use of antibiotics.
Authors and Affiliations
Deborah Adah,Saidu Lawal,sonnie oniye,Adakole Adah,
Keywords
Related Articles
- EP ID EP746826
- DOI -
- Views 31
- Downloads 0
Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
Anti-rabies virus hyperimmune serum was prepared in horses using both inactivated and live attenuated rabies virus (ERA strain) adjuvanted with three different adjuvants including 20% Alhydro gel, 5% Pet gel-A and 20% c...
Isolation and Molecular Characterization of Equine herpes virus-1 from Horses Localized in Giza Governorate, Egypt
Equine herpesvirus-1 (EHV-1) is endemic in many world areas, including Egypt with significant economic losses in the equine industry through causing a high fatality rate among foals associated with respiratory manifestat...
Incidence, Bacterial causes and Antibiotic Resistance Patterns of Urinary Tract Infection in Pet Animals
T The primary goal of the study was to determine the prevalence and various bacterial risks of lower urinary tract infections (UTI) in diseased and seemingly healthy pet animals with and without urine retention whether t...
Assessment of welfare and health of dairy cows under different housing and management systems
This study was conducted in six dairy farms in Beni-Suef Governorate representing the most prevalent systems of housing and management in Egypt to assess the welfare and health of dairy cows kept in these systems. These...
Marek's disease salience in domestic poultry ,gross and a Histopathological study at Mosul city
ABSTRACT Marek's diseases is a contagious disease affected chicken over all the world there are four different form of it and the infected birds may exhibit one or more from ( cutaneous ,visceral , ocular &neural) fo...