Evaluation Of Anti-Rabies Hyperimmune Serum Prepared Using Different Adjuvants
Journal Title: Journal of Applied Veterinary Sciences - Year 2020, Vol 5, Issue 1
Abstract
Anti-rabies virus hyperimmune serum was prepared in horses using both inactivated and live attenuated rabies virus (ERA strain) adjuvanted with three different adjuvants including 20% Alhydro gel, 5% Pet gel-A and 20% calcium phosphate gel. The six vaccine preparations were inoculated subcutaneously in three groups of horses, separately, as each horse group received 4 increased doses of an inactivated vaccine followed by 4 increased doses of a live attenuated vaccine (one dose twice weekly) with the same adjuvant. All prepared vaccine formulae were found to be free from foreign contaminants and safe with no post inoculation abnormal signs in inoculated mice. Monitoring of the levels of exhibited rabies antibodies in the sera of immunized horses using serum neutralization test (SNT) and quantitative ELISA kit revealed that the prepared rabies vaccines with 5% Pet gel A adjuvant induced the highest antibody titers (2048 by SNT and 2.20 by ELISA kit) with expected protection percentage 96.14% followed by those induced by 20% Calcium phosphate gel recording the values of 2048 by SNT and 2.11 by ELISA kit with expected protection percentage 92.2%. Lower values (1024 by SNT; 2.05 by ELISA kit and 89.59% protection percentage) were recorded for the Alhydro gel adjuvanted vaccine. These obtained data reflected the potency of the prepared equine anti-rabies hyperimmune serum (EARHIS) to be used for post exposure treatment in emergency cases and that is the Pet gel A was the best adjuvant to be used.
Authors and Affiliations
Edries, S. M. , Lamees, A, El – Tantawy, Wafaa, R, Abdel Aziz, Ibrahim, H. M, Omaima A.A. Al-Shamandy
Keywords
Related Articles
- EP ID EP679161
- DOI https://dx.doi.org/10.21608/javs.2020.21969.1003
- Views 196
- Downloads 0
Rapid Detection Of Bovine Herpes Virus Type 1 (Bohv-1) In Egyptian Dromedary Camels
Bovine herpesvirus 1 (BoHV-1) is known to cause reproductive disorders in Sudanese camels. Egypt imports about 90% of its camels from Sudan, and the rest from Somalia. The BoHV1 is a viral disease of bovines that can be...
Overview of tuberculosis in Animals in Nigeria
Tuberculosis of animal origin is currently in the increase, especially in the developing countries like Nigeria where animals and man share microenvironment together coupled with lack of proper control measures. The dise...
The relationship between antibiotics resistance and biofilm formation for Escherichia coli isolated from sheep in Nineveh province, Iraq
The study aimed to determine the relationship between antibiotic resistance and the ability to produce biofilm of E.coli isolated from sheep in Nineveh Governorate. One hundred four fecal swabs were collected from health...
Comparative Study of Using Four Types of Avian Egg Yolk on Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
The objective of the current experiment was to study the effect of different avian egg yolks (EYs) on rams epididymal sperms stored at 4 ºC. Sixteen pairs of testes (n=32) were mature Awassi rams of about 1-4 years, sla...
A Survey on Congenital Head Malformations in Calves
Congenital malformations are structural and functional abnormalities that present at birth. Congenital head malformations were present in 231 (22 bovine and 209 buffalo) calves referred to Mansoura veterinary teaching ho...