Enzymatic toxicity induced by lead acetate and its possible reversal
Journal Title: International Journal of Biomedical Research - Year 2015, Vol 6, Issue 11
Abstract
Objective: An important part of male infertility of unknown etiology may be attributed to various environmental and occupational exposures to toxic substances, such as lead. Lead is the known environmental contaminant adversely affecting the male reproductive system in human and experimental animals. The cytotoxic effects of lead on male reproductive system involve the production of reactive oxygen species (ROS) and oxidative damage in tissue. The higher quantities of ROS in testicular tissue detrimentally affected enzymatic activities so as to produce verities of biochemical abnormalities and finally reduced sperm count. Methods: Adult male mice were divided into three groups: control, treated with lead acetated (1.25 mg/kg/day) and recovery group lead acetated (1.25 mg/kg/day) with vitamin E (2 mg/kg/day). Treatments were administered by daily gavage for 45 days. After treatment testis and epididymal weights were recorded and testis was used for the biochemical analysis (acid phosphatase (ACPase), alkaline phosphatase (ALKPase), and glutathione (GSH) and epididymal was used to count sperm. Results: Administration of lead acetate at a dose of 1.25mg/kg body weight for 45 days decreased the activities of all above parameters. Lead exposure resulted in oxidative stress and this was well extrapolated from the increase in lipid peroxidation products (LPP). Coadministration of vitamin E (2 mg/kg/day) with lead acetate restored all the parameters cited above to near the control values. Conclusions: This concludes that vitamin E has beneficial effects against lead acetate induced enzymatic toxicity in testicular tissue of mice.
Authors and Affiliations
Durgesh Nandini Sharma, Lata Bhattacharya
Keywords
Related Articles
- EP ID EP420042
- DOI 10.7439/ijbr.v6i11.2267
- Views 105
- Downloads 0
Antibiotic resistance pattern of enterococcal isolates from patients with urinary tract infection
Background: Enterococci are the most common cause of healthcare associated urinary tract infections. The frequency of isolation of Enterococci from urinary tract of hospitalized patients has risen. Enterococcus is consid...
Diagnostic utility of Plasma Thromboplastin cell block preparation in cytological evaluation of serous effusions
Background: Cell block preparations act as a useful adjunct to smear cytology for categorization of malignant and benign effusions. Plasma thromboplastin cell block technique is simple, requires less time and offers impr...
A Clinical Study of Suppurative Keratitis
Objectives: To find out the aetiological agents and clinical features of suppurative keratitis and to evaluate the adequacy of current therapy available in treating suppurative keratitis. Materials and Methods This stud...
Effect of metformin & myoinositol & life style modification in patients of polycystic ovarian disease (PCOD)
Polycystic ovarian disease (PCOD) is common endocrine disorder. Majority of young females are affected. Insulin resistance along with dysfunction of hypothalamopituitoryadrenal axis is a key etiological factor in develop...
Is Apolipoprotein B and Small, Dense low density lipoprotein a better marker of cardiovascular risk?
The association between low density lipoprotein cholesterol and cardiovascular risk is well established. However, the measurement of the cholesterol content of this lipoprotein incompletely accounts for the risk of cardi...