Epigallocatechin-3-Gallate Induces Apoptosis through Up-regulation of Bax and Down-regulation of Bcl-2 in Prostate Cancer Cell Line
Journal Title: International Journal of Medical Laboratory - Year 2016, Vol 3, Issue 4
Abstract
Background and Aims: Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound from green tea, which its anticancer effects on many types of cancers have been confirmed, but the molecular mechanism by which EGCG induces apoptosis remains unknown. The aim of the present study was to investigate anti-proliferative properties and apoptotic signaling pathway of EGCG on PC3 human prostate cancer cells. Materials and Methods: Cytotoxic effect of EGCG on prostate cancer cell line (PC3) was evaluated by MTT assay. DAPI staining was carried out to determine the morphological appearance of cells. Finally, the expression of Bax and Bcl-2 (apoptosis-regulating genes) were evaluated by quantitative Real-time polymerase chain reaction (PCR). Results: Cytotoxicity evaluations demonstrated that EGCG prevented prostate cancer cells growth in a time and dose depended manner, but the effect of treatment duration is more significant than effect of concentration. Cell growth inhibition was found to be accompanied by nucleus condensation or chromatin fragmentations which are signs of apoptosis, as assessed by DAPI staining. Quantitative Real-time PCR results demonstrated that EGCG causes up-regulation of Bax as a pro-apoptotic protein, and down-regulation of Bcl-2 as an anti-apoptotic protein, thus shifting the Bax/Bcl-2 ratio in favor of apoptosis. Conclusions: It is tempting to suggest that consumption of EGCG could be an effective strategy to inhibit prostate cancer. Our results demonstrated that increase in the ratio of Bax/Bcl-2, is the probable molecular mechanisms through which EGCG induces apoptosis in PC3 cells.
Authors and Affiliations
Hamed Hajipour, Sina Raeisi, Siamak Barghi, Akbar Hasani
Keywords
Related Articles
- EP ID EP212526
- DOI -
- Views 98
- Downloads 0
The Effect of Methanol Extract of Echinops Lasiolepis on TNF-α Production in LPS-activated J774 A.1 Mouse Macrophages
background and Aims: Plants as medicines have always played a vital role in human life. Tumor necrosis factor alpha (TNF)-α is one of the macrophage-derived inflammatory cytokine with pleotropic effects in the infl...
The Relaxant Effect of Carvacrol on Acetylcholine-induced Contraction in Isolated Rat's Ileum
Background and Aims: The antioxidant and anti-inflammatory effects of carvacrol were reported. The aim of the current study was an investigation the relaxant effects of carvacrol on acetylcholine-induced contraction in i...
Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles
Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and met...
Paradoxical Regulation of Copper and Zinc and Changes in Neurogenesis, Alcohol Preference and Salt Appetite in Isolated Male Rats
Background and Aims: Alcohol abuse is an important concern of many societies. Hippocampal neurogenesis regulates abusing drugs in a positive manner. The aim of this study was to identify factors that regulate neurogenesi...
The Peep of Nanotechnology in Reproductive Medicine: A Mini-review
Nanotechnology has opened a new field in medicine as well as in other sciences. The aim of this study was to seek the capability of nanotechnology for the treatment of various reproductive diseases. In this study, we ana...